Intermittent Bolus Feeding Has a Greater Stimulatory Effect on Protein Synthesis in Skeletal Muscle Than Continuous Feeding in Neonatal Pigs
Intermittent Bolus Feeding Has a Greater Stimulatory Effect on Protein Synthesis in Skeletal Muscle Than Continuous Feeding in Neonatal Pigs
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DOI：
10.3945/jn.111.147520
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发表时间：
2011-12-01
影响因子：
4.2
通讯作者：
Davis, Teresa A.
中科院分区：
文献类型：
--
作者：
Gazzaneo, Maria C.;Suryawan, Agus;Davis, Teresa A.
口胃管喂养，使用连续或间歇的小丸喂养，在正常喂养禁忌的婴儿中很常见。为了比较不同饲养策略对肌肉蛋白质合成的影响，在断食过夜后，新生猪每隔4小时口服一次完整配方饲料或连续饲养。在0、24和25.5小时后，研究人员检测了骨骼肌的蛋白质合成率和转化机制。与无饲料猪相比，连续饲养的血浆氨基酸和胰岛素浓度增加幅度最小，并保持不变；然而，脉动式饲粮喂养模式在丸饲猪中被模仿。肌肉蛋白质的合成被喂食刺激，最大的反应发生在丸剂餐后。大剂量而非连续饲喂增加了多体聚集、蛋白激酶B、结节性硬化症复合体2、富含脯氨酸的40 kDa Akt底物、真核起始因子（elF） 4E结合蛋白（4EBP1）和rp S6激酶的磷酸化，并增强了4EBP1 elF4E复合体的解离和elF4E的形成。elF4G配合物与采食剥夺相比（P < 0.051）。胰岛素受体底物-1的激活、哺乳动物雷帕霉素靶蛋白的调控相关蛋白、amp激活的蛋白激酶、真核延伸因子2和elF2 α磷酸化均未受两种喂养方式的影响。这些结果表明，在新生儿中，间歇性大剂量喂养通过激发氨基酸和胰岛素诱导的翻译起始的脉动模式，在更大程度上促进了肌肉蛋白质的合成。中国生物医学工程学报，2011,31(2):555 - 558。
Orogastric tube feeding, using either continuous or intermittent bolus delivery, is common in infants for whom normal feeding is contraindicated. To compare the impact of different feeding strategies on muscle protein synthesis, after withholding food overnight, neonatal pigs received a complete formula orally as a bolus feed every 4 h or were continuously fed. Protein synthesis rate and translational mechanisms in skeletal muscle were examined after 0, 24, and 25.5 h. Plasma amino acid and insulin concentrations increased minimally and remained constant in continuously fed compared to feed-deprived pigs; however, the pulsatile meal feeding pattern was mimicked in bolus-fed pigs. Muscle protein synthesis was stimulated by feeding and the greatest response occurred after a bolus meal. Bolus but not continuous feeds increased polysome aggregation, the phosphorylation of protein kinase B, tuberous sclerosis complex 2, proline-rich Akt substrate of 40 kDa, eukaryotic initiation factor (elF) 4E binding protein (4EBP1), and rp S6 kinase and enhanced dissociation of the 4EBP1 elF4E complex and formation of the elF4E. elF4G complex compared to feed deprivation (P < 0.051. Activation of insulin receptor substrate-1, regulatory associated protein of mammalian target of rapamycin, AMP-activated protein kinase, eukaryotic elongation factor 2, and elF2 alpha phosphorylation were unaffected by either feeding modality. These results suggest that in neonates, intermittent bolus feeding enhances muscle protein synthesis to a greater extent than continuous feeding by eliciting a pulsatile pattern of amino acid- and insulin-induced translation initiation. J. Nutr. 141: 2152-2158, 2011.