Infection with High-risk HPV Types among Female Sex Workers in Northern Vietnam
Infection with High-risk HPV Types among Female Sex Workers in Northern Vietnam
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越南北部女性性工作者的高危型 HPV 感染情况
DOI：
10.1002/jmv.23456
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发表时间：
2013
期刊：
影响因子：
12.7
通讯作者：
and Ichimura H.
中科院分区：
文献类型：
--
作者：
Hoang HT;Ishizaki A;Nguyen CH;Tran VT;Matsushita K;Saikawa K;Hosaka N;Pham HV;Bi X;Ta VT;Pham TV;and Ichimura H.
目前正在使用针对两种高危型人乳头瘤病毒（HPV）类型（HPV-16和HPV-18）的疫苗，对预防这些HPV类型的感染和随后的宫颈癌具有很高的疗效。然而，循环HPV类型可能因地理和种族而异。本研究的目的是调查越南北方女性性工作者中HPV类型的患病率以及HPV类型与宫颈细胞学异常之间的关系。2009年在河内和海丰的两个卫生中心收集了281名女性性工作者的宫颈拭子和血浆样本。用原始和改良的GP 5 +/6+引物对HPV L1基因进行PCR扩增。通过微阵列系统GeneSquare（KURABO）和/或克隆测序对扩增的PCR产物进行基因分型。在281名妇女中，139名（49.5%）HPV DNA阳性。在HPV阳性样本中，确定了339种菌株和29种不同类型。分别在85名（61.2%）和124名（89.2%）女性中发现了多型和高危型HPV感染。最常见的基因型是HPV-52，其次是HPV-16，HPV-18和HPV-58。在3.2%（9/281）的妇女中检测到异常宫颈细胞学，所有这些样本均为HPV-DNA阳性。年龄≤25岁、HIV感染与HPV感染呈正相关，吸烟与HPV感染呈负相关。这些结果表明，HPV-52在越南北方的女性性工作者中最普遍，其中大多数人的宫颈细胞学检查正常。这些信息可能对制定越南的疫苗接种策略很重要。J. Med. Virol. 85：288-294，2013. © 2012 Wiley Periodicals，Inc.
Vaccines against two high‐risk human papillomavirus (HPV) types, HPV‐16, and HPV‐18, are in use currently, with high efficacy for preventing infections with these HPV types and consequent cervical cancers. However, circulating HPV types can vary with geography and ethnicity. The aim of this study was to investigate the prevalence of HPV types and the association between HPV types and abnormal cervical cytology among female sex workers in Northern Vietnam. Cervical swabs and plasma samples were collected from 281 female sex workers at two health centers in Hanoi and Hai Phong in 2009. The HPV L1 gene was amplified by PCR using original and modified GP5+/6+primers. Amplified PCR products were genotyped by the microarray system GeneSquare (KURABO) and/or clonal sequencing. Of the 281 women, 139 (49.5%) were positive for HPV DNA. Among the HPV‐positive samples, 339 strains and 29 different types were identified. Multiple‐type and high risk‐type HPV infections were found in 85 (61.2%) and 124 (89.2%) women, respectively. The most common genotype was HPV‐52, followed by HPV‐16, HPV‐18, and HPV‐58. Abnormal cervical cytology was detected in 3.2% (9/281) of the women, and all of these samples were positive for HPV‐DNA. Age ≤25 years and infection with human immunodeficiency virus were associated positively with HPV infection among the women while ever smoking was associated negatively. These results show that HPV‐52 is most prevalent among female sex workers in Northern Vietnam, most of whom had normal cervical cytology. This information may be important for designing vaccination strategies in Vietnam. J. Med. Virol. 85:288–294, 2013. © 2012 Wiley Periodicals, Inc.