SYNTHESIS AND CHARACTERIZATION OF A POLYVALENT ESCHERICHIA-COLI O-POLYSACCHARIDE TOXIN-A CONJUGATE VACCINE
SYNTHESIS AND CHARACTERIZATION OF A POLYVALENT ESCHERICHIA-COLI O-POLYSACCHARIDE TOXIN-A CONJUGATE VACCINE
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DOI：
10.1016/0264-410x(94)00009-c
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发表时间：
1995-04-01
期刊：
影响因子：
5.5
通讯作者：
FURER, E
中科院分区：
文献类型：
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作者：
CRYZ, SJ;QUE, JO;FURER, E
12价大肠杆菌O-多糖（O-PS）-毒素A结合疫苗是由以下血清型的脂多糖（LPS）制成的无热原、低分子量的O-PS。以己二酸二酰肼为间隔分子，碳二亚胺为偶联剂，将单个O-PS与铜绿假单胞菌毒素A共价偶联。以重量计，最终的多价疫苗由43%的O-PS和57%的毒素A组成。该疫苗在标准动物试验中无毒、无热原。免疫家兔后，抗LPS免疫球蛋白G（IgG）抗体滴度显著升高（6-74倍）。当被动转移到小鼠时，免疫土耳其兔IgG赋予统计学显著性（p
A 12-valent Escherichia coli O-polysaccharide (O-PS)-toxin A conjugate vaccine was formulated Nonpyrogenic, low-molecular-weight O-PS, was derived from lipopolysaccharides (LPS) of the following serotypes: 01, 02, 04, 06, 07, 08, 012 015, 016, 018, 025, and 075. Individual O-PS were covalently coupled to Pseudomonas aeruginosa toxin A using adipic acid dihydrazide as a spacer molecule and carbodiimide as a coupling agent. On a weight basis, the final multivalent vaccine was composed of 43% O-PS and 57% toxin A. The vaccine was nontoxic and nonpyrogenic in standard animal tests. Immunization of rabbits engendered a marked rise (6-74-fold) in anti-LPS immunoglobulin G (IgG) antibody titers. When passively transferred to mice, immune turze rabbit IgG conferred statistically significant (p