microRNA-133 inhibits cell proliferation, migration and invasion in prostate cancer cells by targeting the epidermal growth factor receptor
microRNA-133 inhibits cell proliferation, migration and invasion in prostate cancer cells by targeting the epidermal growth factor receptor
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DOI：
10.3892/or.2012.1711
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发表时间：
2012-06-01
期刊：
影响因子：
4.2
通讯作者：
Zhang, Wei
中科院分区：
文献类型：
--
作者：
Tao, Jun;Wu, Deyao;Zhang, Wei
已有研究表明，前列腺癌细胞中EGFR的表达调控主要是在转录水平。MicroRNA-133(miR-133)是一种肌肉特异性miRNA，可调节成肌细胞分化，参与多种肌源性疾病。最近，有报道称miR-133还参与了其他肿瘤，如膀胱癌、食道癌等，并可能调节这些癌细胞的细胞运动。在本研究中，我们检测了miR-133在两个激素不敏感的前列腺癌细胞系中的表达和作用。定量RT-PCR检测miR-133a和miR-133b的表达。转染miR-133a和miR-133b后，分别对DU145和PC3细胞进行细胞活力测定、荧光素酶活性测定、蛋白质印迹分析、细胞迁移和侵袭实验。在这项研究中，我们发现miR-133a和miR-133b在两个激素不敏感的前列腺癌细胞系PC3和DU145中的表达达到了检测极限。异位表达miR-133抑制了这些细胞的增殖、迁移和侵袭。我们还提供了miR-133可能靶向EGFR的第一个证据。我们的研究首次揭示了miR-133在两种激素非依赖性前列腺癌细胞系中的功能作用。这些结果可能会增加我们对前列腺癌进展的分子基础的了解。
It has been shown that regulation of EGFR expression in prostate cancer cells is mostly at the transcriptional level. microRNA-133 (miR-133) has long been recognized as a muscle-specific miRNA which may regulate myoblast differentiation and participate in many myogenic diseases. Recently, it has been reported that miR-133 is also involved in other tumors, such as bladder cancer, esophageal cancer and may regulate cell motility in these cancer cells. In the present study, we examined the expression and effects of miR-133 in two hormone-insensitive prostate cancer cell lines. The expression of miR-133a and miR-133b were analyzed by quantitative RT-PCR. After transfection of miR-133a and miR-133b, cell viability assay, luciferase assay, western blot analysis, cell migration and invasion assay were conducted in DU145 and PC3 cells. In this study, we showed that miR-133a and miR-133b are expressed at the detection limit in two hormone-insensitive prostate cancer cell lines, PC3 and DU145. Ectopic expression of miR-133 inhibited cell proliferation, migration and invasion in these cells. We also provide the first evidence that miR-133 may target EGFR. Our study provided the first glimpse of the functional role of miR-133 in two hormone-independent prostate cancer cell lines. These results may add to our knowledge on the molecular basis of prostate cancer progression.