Dual effect of isoprostanes on the release of [3H]D-aspartate from isolated bovine retinae: role of arachidonic acid metabolites.
Dual effect of isoprostanes on the release of [3H]D-aspartate from isolated bovine retinae: role of arachidonic acid metabolites.
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异前列烷对离体牛视网膜释放 [3H]D-天冬氨酸的双重作用：花生四烯酸代谢物的作用。
DOI：
10.1007/s11064-004-9694-3
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发表时间：
2005
影响因子：
4.4
通讯作者：
Ohia,SunnyE
中科院分区：
文献类型：
--
作者：
Opere,CatherineA;Zheng,WeiDong;Huang,Jifan;Adewale,Adeniran;Kruglet,Michael;Ohia,SunnyE
本文研究了8-异前列腺素对钾(K+)去极化引起的牛视网膜[~3H]D-天冬氨酸释放的影响。分离的牛视网膜用灌流法研究K+诱发的[~3H]D-天冬氨酸的释放。低浓度的8-isPGF2α(1-100 nM)抑制K+诱导的[~3H]D-天冬氨酸溢出，而高浓度的8-异前列腺素(100 nM-30μM)则增强K+诱导的[~3H]D-天冬氨酸溢出。血栓素受体激动剂U-46619模拟8-异前列腺素F2α的兴奋作用，并被血栓素受体拮抗剂SQ 29,548(10μM)阻断。用环氧合酶(COX)抑制剂预先处理组织，氟比洛芬发现高浓度8-α(1-30μM)对[~3H]D-天冬氨酸释放的抑制作用可被AH6809(10μM)减弱。综上所述，8-isPGF2α对K+诱导的牛视网膜[~3H]D-天冬氨酸的释放具有双重调节作用。8-异PGF2α的抑制作用是通过激活EP1/EP2受体实现的，而兴奋作用是通过激活血栓素A受体实现的。
The effect of 8-isoprostanes on potassium (K+)-depolarization-evoked release of [3H]D-aspartate from bovine isolated retinae was investigated. Isolated bovine retinae were prepared for studies of K+-evoked release of [3H]D-aspartate using the Superfusion Method. Low concentrations of 8-isoPGF2α(1–100 nM) inhibited whereas higher concentrations of this 8-isoprostane (100 nM–30 μM) enhanced K+-induced [3H]D-aspartate overflow. The excitatory effect of 8-isoPGF2αwas mimicked by thromboxane receptor agonist, U-46619 and blocked by thromboxane receptor antagonist, SQ 29,548 (10 μM). Pretreatment of tissues with the cyclooxygenase (COX) inhibitor, flurbiprofen unmasked an inhibitory effect of high concentrations of 8-isoPGF2α(1–30 μM) on [3H]D-aspartate release that was attenuated by AH 6809 (10 μM). In conclusion, 8-isoPGF2αexhibits a dual regulatory effect on K+-induced [3H]D-aspartate release in isolated bovine retinae. The inhibitory action caused by 8-isoPGF2αis due to the activation of EP1/EP2receptors while the excitatory effects are due to the activation of thromboxane receptors.