Self-assembly of a dual functional bioactive peptide amphiphile incorporating both matrix metalloprotease substrate and cell adhesion motifs.
Self-assembly of a dual functional bioactive peptide amphiphile incorporating both matrix metalloprotease substrate and cell adhesion motifs.
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DOI：
10.1039/c5sm00459d
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发表时间：
2015-04
期刊：
影响因子：
3.4
通讯作者：
A. Dehsorkhi;R. Gouveia;Andrew M. Smith;I. Hamley;V. Castelletto;C. Connon;M. Reza;J. Ruokolainen
中科院分区：
文献类型：
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作者：
A. Dehsorkhi;R. Gouveia;Andrew M. Smith;I. Hamley;V. Castelletto;C. Connon;M. Reza;J. Ruokolainen
我们描述了一种生物活性脂肽，它结合了通过模板-细胞-环境反馈相互作用促进活细胞黏附和随后的自我分离的能力。这种自组装肽两亲性包括一个含有二烯的十六烷基脂链(C16e)，连接到基质金属蛋白酶可切割的序列Thr-Pro-Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln，并与细胞附着和信号基序Arg-Gly-Asp-Ser相连。生物物理表征表明，PA在临界聚集浓度(CAC)以上自组装成直径3 nm的球形胶束。此外，当在5-150纳米(远低于CAC)的溶液中使用时，PA能够形成薄膜涂层，为人角膜成纤维细胞的附着和生长提供稳定的表面。此外，这些涂层被证明对附着的细胞内源性表达的金属蛋白水解酶敏感，从而在不影响其活性的情况下诱导细胞的受控分离。因此，这种材料构成了一种新型的多功能涂层，可用于组织工程的基础和临床应用。
We describe a bioactive lipopeptide that combines the capacity to promote the adhesion and subsequent self-detachment of live cells, using template-cell-environment feedback interactions. This self-assembling peptide amphiphile comprises a diene-containing hexadecyl lipid chain (C16e) linked to a matrix metalloprotease-cleavable sequence, Thr-Pro-Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln, and contiguous with a cell-attachment and signalling motif, Arg-Gly-Asp-Ser. Biophysical characterisation revealed that the PA self-assembles into 3 nm diameter spherical micelles above a critical aggregation concentration (cac). In addition, when used in solution at 5-150 nM (well below the cac), the PA is capable of forming film coatings that provide a stable surface for human corneal fibroblasts to attach and grow. Furthermore, these coatings were demonstrated to be sensitive to metalloproteases expressed endogenously by the attached cells, and consequently to elicit the controlled detachment of cells without compromising their viability. As such, this material constitutes a novel class of multi-functional coating for both fundamental and clinical applications in tissue engineering.