LATERAL MOBILITY OF INTEGRAL MEMBRANE-PROTEINS IS INCREASED IN SPHEROCYTIC ERYTHROCYTES
LATERAL MOBILITY OF INTEGRAL MEMBRANE-PROTEINS IS INCREASED IN SPHEROCYTIC ERYTHROCYTES
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DOI：
10.1038/285510a0
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发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
KOPPEL, DE
中科院分区：
文献类型：
--
作者：
SHEETZ, MP;SCHINDLER, M;KOPPEL, DE
细胞膜中糖蛋白分布和横向流动性的改变可以为几种膜相关现象提供跨膜信号1-3。跨膜事件的控制归因于膜下蛋白基质（或“细胞骨架”）和膜糖蛋白 4-7 之间的相互作用。这种相互作用的结果是生物膜中蛋白质横向扩散的差异抑制。事实上，膜蛋白横向扩散系数的测量通常产生的值 8-12 远低于流体双层中无阻碍扩散的预测值 13,14。小鼠球形红细胞缺乏正常红细胞膜基质的主要成分15（由血影蛋白、肌动蛋白、条带 4.1 和 4.9（参考文献 16）组成，按照 Fairbankset al.17 的命名法），提供了一个独特的系统来直接评估基质对蛋白质横向迁移率的影响。在使用光漂白后荧光重新分布 (FRAP)18 技术的改进后，我们在此报道膜蛋白在球形红细胞中的扩散速度比在正常小鼠红细胞中快约 50 倍。
Alterations of glycoprotein distribution and lateral mobility in cell membranes can provide transmembrane signals for several membrane-related phenomena1–3. Control of the transmembranous events has been ascribed to interaction between submembranous protein matrices (or ‘cytoskeletons’) and membrane glycoproteins4–7. A consequence of such interaction would be differential inhibition of protein lateral diffusion in biological membranes. Measurements of the lateral diffusion coefficients of membrane proteins, in fact, have generally yielded values8–12much less than were predicted for unhindered diffusion in a fluid bilayer13,14. The mouse spherocytic erythrocyte, which lacks the major components of the normal erythrocyte membrane matrix15(composed of spectrin, actin, bands 4.1 and 4.9 (ref. 16), in the nomenclature of Fairbankset al.17), provides a unique system for a direct evaluation of the effect of the matrix on protein lateral mobility. After using a modification of the technique of fluorescence redistribution after photobleaching (FRAP)18, we report here that membrane proteins diffuse about 50 times faster in spherocytic than in normal mouse erythrocytes.