Novel Animal Pole-Enriched Maternal mRNAs are Preferentially Expressed in Neural Ectoderm
Novel Animal Pole-Enriched Maternal mRNAs are Preferentially Expressed in Neural Ectoderm
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DOI：
10.1002/dvdy.24082
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发表时间：
2014-03-01
影响因子：
2.5
通讯作者：
Moody, Sally A.
中科院分区：
文献类型：
--
作者：
Grant, Paaqua A.;Yan, Bo;Moody, Sally A.
背景：许多动物在合子转录开始之前利用母体mRNA来预定型胚胎。在非洲爪蟾，植物因素指定的种系，内胚层，和背轴，但很少有研究表明动物丰富的母体mRNA的作用。因此，我们进行了一个微阵列分析，以确定新的母亲的成绩单丰富的8-细胞阶段的动物blastomeres.Results：我们确定了39个mRNA分离的8-细胞动物卵裂球，是4倍富集相比，植物极mRNA。我们确定了其中14个未知功能。我们通过qRT-PCR验证了8/14个基因的微阵列结果，通过原位杂交测定验证了14/14个基因的微阵列结果。由于还没有发育功能的报道，我们提供了14个基因中每一个的表达模式。每一种都在未受精卵的动物半球、8细胞动物卵裂球中表达，并且在囊胚动物帽外胚层、原肠胚外胚层和神经外胚层、神经嵴（和衍生物）和颅基板（和衍生物）中弥散表达。它们在尾芽的中胚层和内胚层组织中有不同的后期表达。结论：新型动物富集的母体mRNA优先在外胚层衍生物，特别是神经外胚层中表达。然而，它们后来在其他种系的衍生物中表达。发展动力学243：478-496，2014年。(c)2013 Wiley Periodicals，Inc.
Background: Many animals utilize maternal mRNAs to pre-pattern the embryo before the onset of zygotic transcription. In Xenopus laevis, vegetal factors specify the germ line, endoderm, and dorsal axis, but there are few studies demonstrating roles for animal-enriched maternal mRNAs. Therefore, we carried out a microarray analysis to identify novel maternal transcripts enriched in 8-cell-stage animal blastomeres.Results: We identified 39 mRNAs isolated from 8-cell animal blastomeres that are >4-fold enriched compared to vegetal pole mRNAs. We characterized 14 of these that are of unknown function. We validated the microarray results for 8/14 genes by qRT-PCR and for 14/14 genes by in situ hybridization assays. Because no developmental functions are reported yet, we provide the expression patterns for each of the 14 genes. Each is expressed in the animal hemisphere of unfertilized eggs, 8-cell animal blastomeres, and diffusely in blastula animal cap ectoderm, gastrula ectoderm and neural ectoderm, neural crest (and derivatives) and cranial placodes (and derivatives). They have varying later expression in some mesodermal and endodermal tissues in tail bud through larval stages. Conclusions: Novel animal-enriched maternal mRNAs are preferentially expressed in ectodermal derivatives, particularly neural ectoderm. However, they are later expressed in derivatives of other germ layers. Developmental Dynamics 243:478-496, 2014. (c) 2013 Wiley Periodicals, Inc.