Capillary electrochromatography of biomolecules with on-line electrospray ionization and time-of-flight mass spectrometry.
Capillary electrochromatography of biomolecules with on-line electrospray ionization and time-of-flight mass spectrometry.
复制标题
采用在线电喷雾电离和飞行时间质谱法对生物分子进行毛细管电色谱分析。
DOI：
10.1016/s0021-9673(98)00817-6
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Banks,JF
中科院分区：
文献类型：
--
作者：
Choudhary,G;Horváth,C;Banks,JF
毛细管电色谱（CEC）是液相色谱和毛细管电泳的混合物。将毛细管区带电泳的高峰效率与高效液相色谱的多功能性和上样能力联合收割机相结合，有望产生另一种高效的MS兼容色谱系统。本文探讨了CEC与电喷雾离子化和飞行时间质谱联用在生化分析中的潜力。本研究中使用的填充柱在出口处逐渐变细以保留填充材料，从而避免了对出口玻璃料的需要。采用电驱动溶剂梯度反相色谱分离苯硫海因（PTH）-氨基酸，飞行时间（TOF）质谱仪作为检测器的CEC柱流出物。CEC操作参数，如梯度形状，柱长，和电场，从PTH-氨基酸的标准混合物的分离和MS检测的分析结果的影响进行了研究。研究了鞘层流速、鞘层组成和质谱采集速率对电喷雾飞行时间质谱的影响。
Capillary electrochromatography (CEC) is considered a hybrid of liquid chromatography and capillary electrophoresis. It is expected to combine the high peak efficiency of capillary zone electrophoresis with the versatility and loading capacity of HPLC to bring about another high-performance MS-compatible chromatographic system. This paper explores the potential of CEC coupled with the electrospray ionization and time-of-flight mass spectrometry in biochemical analysis. The packed columns used in this study were tapered at the outlet to retain the packing material, thereby obviating the need for an outlet frit. Electrosmotically driven solvent gradients were employed for the separation of phenylthiohydantoin (PTH)-amino acids by reversed-phase chromatography, and a time-of-flight (TOF) mass spectrometer was employed as the detector for the CEC column effluent. The effect of CEC operating parameters, such as gradient shape, column length, and electric field, on the analytical results from the separation and MS detection of a standard mixture of PTH-amino acids was investigated. Particular attention was paid to the effect of sheath flow-rate, sheath composition and mass spectra acquisition rate on the performance of the electrospray TOF-MS.