Characterization and biological effect of Buenos Aires urban air particles on mice lungs
Characterization and biological effect of Buenos Aires urban air particles on mice lungs
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DOI：
10.1016/j.envres.2007.04.009
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发表时间：
2007-11-01
影响因子：
8.3
通讯作者：
Tasat, Deborah Ruth
中科院分区：
文献类型：
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作者：
Martin, Susana;Dawidowski, Laura;Tasat, Deborah Ruth
暴露于环境空气颗粒物（PM）水平增加与心肺发病率和死亡率增加有关。它与不良健康影响的关联以及尚不清楚的作用机制在全世界引起关注。我们的目标是分析空气PM从布宜诺斯艾利斯市中心（UAP-BA），并评估其对正常气道的生物影响。我们在短期暴露的小鼠模型中研究了UAP-BA鼻内滴注的炎症反应。我们分析了UAP-BA的形态，通过扫描电子显微镜和其特征在于颗粒的化学组成，通过能量色散X射线分析和毛细管气相色谱。我们通过组织形态计量学和组织化学方法评价了肺的变化。关于尺寸、表面积和分布，UAP-BA被证明是小的球形超细颗粒：游离的、成簇的和与基质相关的。这些颗粒含有多环芳烃、多氯联苯，几乎没有金属痕迹。在组织学上，UAP-BA引起吞噬细胞募集，气隙减少，粘液PAS阳性细胞增多，弹性纤维网络不完整。我们的研究结果表明，UAP-BA导致呼吸道产生炎症，反过来，可能会导致组织损伤或器官功能障碍，并可能有助于肺部疾病的发病机制的不良生物学效应。(C)2007年爱思唯尔公司All rights reserved.
Exposure to increased levels of ambient air particulate matter (PM) is associated with increased cardiopulmonary morbidity and mortality. Its association with adverse health effects and the still unclear mechanisms of action are of concern worldwide. Our objective was to analyze air PM from downtown Buenos Aires (UAP-BA), and evaluate its biological impact on normal airways. We studied the inflammatory response to intranasal instillation of UAP-BA in a short-term-exposure mouse model. We analyzed UAP-BA morphology by scanning electron microscopy and characterized particle chemical composition by energy dispersive X-ray analysis and capillary gas chromatography. We evaluated lung changes by histomorphometry and histochemical methods. Regarding size, surface area and distribution, UAP-BA proved to be small spherical ultrafine particles: free, in clusters and associated to a matrix. The particles contained polycyclic aromatic hydrocarbons, polychlorinated biphenyls and almost no metal traces. Histologically, UAP-BA induced the recruitment of phagocytes, a reduction in air spaces, an increase in mucous PAS positive cells and weak incomplete elastic fiber network. Our results demonstrate that UAP-BA causes adverse biological effects on the respiratory tract generating inflammation that, in turn, may cause tissue injury or organ dysfunction and may contribute to the pathogenesis of lung diseases. (C) 2007 Elsevier Inc. All rights reserved.