Haemocytes of the clam Tapes philippinarum (Adams & Reeve, 1850):: morphofunctional characterisation
Haemocytes of the clam Tapes philippinarum (Adams & Reeve, 1850):: morphofunctional characterisation
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DOI：
10.1006/fsim.2000.0282
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发表时间：
2000-11-01
影响因子：
4.7
通讯作者：
Ballarin, L
中科院分区：
文献类型：
--
作者：
Cima, F;Matozzo, V;Ballarin, L
Tapes philippinarum是太平洋的一种双壳软体动物，成功地进口到亚得里亚海北部(欧洲)供人类食用。为了更好地了解其与类似原生物种相比的相当大的适应能力，通过建立短期细胞培养(在25摄氏度下培养60分钟)，对其血细胞进行了形态功能表征。不同的细胞化学染色方法鉴定出四种细胞类型：粒细胞(48.05%+/-1.43)、透明细胞(32.18%+/-0.99)、造血细胞(18.97%+/-0.63)和浆细胞(0.8%+/-0.19)。嗜碱性粒细胞、嗜中性粒细胞和嗜酸性粒细胞具有不同染色亲和力的胞质颗粒。这样的颗粒与中性FED染色强烈，与溶酶体一致。酵母菌刺激后，主要检测到水解酶和氧化酶。粒细胞和透明细胞均呈碱性磷酸酶、非特异性酯酶、过氧化物酶和细胞色素C氧化酶阳性，而只有粒细胞呈β-葡萄糖醛酸酶、酸性酯酶和芳基硫酸酯酶阳性。两种细胞对酵母菌均有吞噬活性，血浆有调理作用。此外，呼吸爆发伴随着吞噬和超氧阴离子的产生，通过硝基蓝四氮唑处理后的福马赞细胞质沉积可以辨认出来。造血细胞是一种小的未分化细胞，由于其形态和抗CD34抗体阳性，显示出干细胞的典型特征。浆液细胞可能起源于Keber腺体，属于另一种分化途径，含有非硫酸盐粘多糖，在早期防御机制中发挥重要作用，参与凝块的形成。(C)2000年学术出版社。
Tapes philippinarum is a bivalve mollusc of the Pacific Ocean, successfully imported for human consumption into the northern Adriatic Sea (Europe). For better knowledge of its considerable adaptive ability in comparison with similar autochthonous species, a morpho-functional characterisation of its haemocytes was carried out with the establishment of short-term cell cultures (60 min at 25 degrees C). Various methods of cytochemical staining identified four cell types in the haemolymph: granulocytes (48.05% +/- 1.43), hyalinocytes (32.18% +/- 0.99), haemoblasts (18.97% +/- 0.63) and serous cells (0.8% +/- 0.19). The granulocytes, possessing cytoplasmic granules with differing dye affinity, included basophils, neutrophils and acidophile. Such granules stained vitally with Neutral Fed, and correspond to lysosomes. Hydrolytic and oxidative enzymes were mainly detectable after stimulation in the presence of yeast cells. Both granulocytes and hyalinocytes were positive for alkaline phosphatase, non-specific esterase, peroxidase, and cytochrome C oxidase, whereas only granulocytes were positive for beta -glucuronidase, acid esterase, and arylsulphatase. Both cell types were competent phagocytes towards yeast and plasma had an opsonising effect. Moreover, the respiratory burst accompanied phagocytosis with superoxide anion production, recognisable through cytoplasmic deposits of formazan after treatment with nitro blue tetrazolium. Haemoblasts were small undifferentiated cells which, due to their morphology and positivity to the anti-CD34 antibody, show the typical features of stem cells. Serous cells, probably arising from Keber's gland and belonging to another differentiation pathway, contained non-sulphate acid mucopolysaccharides and play an important role in early defence mechanisms, taking part in the formation of clots. (C) 2000 Academic Press.