GENETIC-TRANSFORMATION OF AN ENTOMOPATHOGENIC NEMATODE BY MICROINJECTION
GENETIC-TRANSFORMATION OF AN ENTOMOPATHOGENIC NEMATODE BY MICROINJECTION
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DOI：
10.1006/jipa.1995.1103
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发表时间：
1995-11-01
影响因子：
3.4
通讯作者：
GAUGLER, R
中科院分区：
文献类型：
--
作者：
HASHMI, S;HASHMI, G;GAUGLER, R
我们报告了第一个成功的昆虫病原线虫转化。外源基因导入异小杆线虫HP 88通过显微注射使用载体携带秀丽隐杆线虫基因编码的辊表型和16-kDa的热休克蛋白（hsp 16）基因。将lacZ插入框架中到hsp 16中的平移融合在身体肌肉组织、皮下组织和咽肌中表达。hsp 16/lacZ转基因的转录导致可检测水平的β-半乳糖苷酶的快速合成。该研究为昆虫病原线虫的遗传修饰开辟了新的途径。(C)出版社：Academic Press
We report the first successful transformation of an entomopathogenic nematode. Foreign genes were introduced in Heterorhabditis bacteriophora HP88 by microinjection using vectors carrying the Caenorhabditis elegans genes coding for the roller phenotype and 16-kDa heat shock protein (hsp16) gene. A translational fusion made by inserting lacZ in frame into hsp16 was expressed in the body musculature, hypodermis, and pharyngeal muscles. The transcription of the hsp16/lacZ transgenes resulted in the rapid synthesis of detectable levels of beta-galactosidase. This research opens new avenues for genetic modification of entomopathogenic nematodes. (C) 1995 Academic Press, Inc.