Successful treatment by double-filtration plasmapheresis of a patient with bullous pemphigoid:: effects in vivo on transcripts of several genes for chemokines and cytokines in peripheral blood mononuclear cells
Successful treatment by double-filtration plasmapheresis of a patient with bullous pemphigoid:: effects in vivo on transcripts of several genes for chemokines and cytokines in peripheral blood mononuclear cells
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DOI：
10.1046/j.1365-2133.2003.05233.x
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发表时间：
2003-03-01
影响因子：
10.3
通讯作者：
Fujiwara, S
中科院分区：
文献类型：
--
作者：
Hatano, Y;Katagiri, K;Fujiwara, S
大疱性类天疱疮(BP)的发病机制涉及多种细胞因子和趋化因子。双滤过血浆置换(DFPP)是治疗BP的有效方法，但其作用机制尚不清楚。应用半定量逆转录聚合酶链式反应，检测了一例BP患者在DFPP治疗前后新鲜分离的外周血单个核细胞中各种细胞因子和趋化因子的转录水平。DFPP手术4次。治疗前IL-8、巨噬细胞炎症蛋白(MIP)-1α和IL-5的相对转录水平以及IL-4和干扰素(干扰素)-γ的相对转录水平的比值均高于健康对照组，并随着病变程度的减轻而降低。肿瘤坏死因子-α和IL-4的相对转录水平也随着病变的消退而降低，尽管它们与健康个体的相应水平相似或更低。当皮疹复发时，IL-8、MIP-1α、RANTES(受激活调节的正常T细胞表达和分泌)、IL-2、干扰素-γ和肿瘤坏死因子-α的相对转录水平明显高于复发前，而IL-4和IL-5的相对水平没有增加。IL-8、MIP-1α、TNF-α和IL-2的相对转录水平在每个DFPP结束时和四种治疗后均低于治疗开始时。提示单核细胞产生的细胞因子和趋化因子在BP的发病机制中起重要作用，其表达的调节可能参与DFPP对BP的治疗作用。
The involvement of various cytokines and chemokines has been reported in the pathogenesis of bullous pemphigoid (BP). Double-filtration plasmapheresis (DFPP) is an effective treatment for BP but the mechanism of action remains unclear. Using semiquantitative reverse transcription-polymerase chain reaction, we examined levels of transcripts for various cytokines and chemokines in freshly isolated peripheral blood mononuclear cells in a patient with BP before and after DFPP treatment. DFPP was performed four times. Relative levels of transcripts for interleukin (IL)-8, macrophage inflammatory protein (MIP)-1alpha and IL-5, and the ratio of relative levels of transcripts for IL-4 and interferon (IFN)-gamma, were higher, before treatment, than in healthy controls, and decreased when the extent of the lesions was reduced. Relative levels of transcripts for tumour necrosis factor (TNF)-alpha and IL-4 also decreased with regression of lesions, although they were similar to or lower than the corresponding levels in healthy individuals. When eruptions recurred, relative levels of transcripts for IL-8, MIP-1alpha, RANTES (regulated upon activation normal T cell expressed and secreted), IL-2, IFN-gamma and TNF-alpha were very much higher than those prior to the recurrence, while relative levels of mRNAs for IL-4 and IL-5 did not increase. Relative levels of transcripts for IL-8, MIP-1alpha, TNF-alpha and IL-2 were lower at the end of each individual DFPP and after the four treatments than at the beginning of treatment. Our observations suggest that cytokines and chemokines produced in mononuclear cells play important roles in the pathogenesis of BP and that regulation of their expression might be involved in the therapeutic effects of DFPP in BP.