Folate-receptor-mediated delivery of InP quantum dots for bioimaging using confocal and two-photon microscopy
Folate-receptor-mediated delivery of InP quantum dots for bioimaging using confocal and two-photon microscopy
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DOI：
10.1021/ja051455x
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发表时间：
2005-08-17
影响因子：
15
通讯作者：
Prasad, PN
中科院分区：
文献类型：
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作者：
Bharali, DJ;Lucey, DW;Prasad, PN
报道了一种合成高度单分散亲水性 InP-ZnS 纳米晶体的新方法及其作为活细胞成像发光探针的用途。采用新方法制备疏水性 InP-ZnS 纳米晶体，在总反应时间内 6-8 小时即可产生高质量的发光核壳纳米晶体。然后，通过仔细操作这些钝化纳米晶体的表面，制备出具有高光稳定性的叶酸共轭纳米晶体（叶酸-QD）的水分散体。通过使用共聚焦显微镜，我们证明了叶酸缀合量子点通过受体介导递送到叶酸受体阳性细胞系（例如 KB 细胞）中。孵育 6 小时后，这些叶酸-QD 倾向于在 KB 细胞的多泡体中积累。通过与叶酸受体阴性细胞系（例如 A549）中这些颗粒的摄取进行比较，证实了受体介导的递送。还证明了这些粒子的有效双光子激发以及使用这些粒子的双光子成像。这些 InP-ZnS 纳米颗粒的使用及其有效的双光子激发可能对未来体内研究的深层组织成像有用。
A novel method for the synthesis of highly monodispersed hydrophillic InP-ZnS nanocrystals and their use as luminescence probes for live cell imaging is reported. Hydrophobic InP-ZnS nanocrystals are prepared by a new method that yields high-quality, luminescent core-shell nanocrystals within 6-8 h of total reaction time. Then by carefully manipulating the surface of these passivated nanocrystals, aqueous dispersions of folate-conjugated nanocrystals (folate-QDs) with high photostability are prepared. By use of confocal microscopy, we demonstrate the receptor-mediated delivery of folic acid conjugated quantum dots into folate-receptor-positive cell lines such as KB cells. These folate-QDs tend to accumulate in multivescicular bodies of KB cells after 6 h of incubation. Receptor-mediated delivery was confirmed by comparison with the uptake of these particles in folate-receptor-negative cell lines such as A549. Efficient two-photon excitation of these particles and two-photon imaging using these particles are also demonstrated. The use of these InP-ZnS nanoparticles and their efficient two-photon excitation can be potentially useful for deep tissue imaging for future in vivo studies.