Adding pyrrolysine to the Escherichia coli genetic code
Adding pyrrolysine to the Escherichia coli genetic code
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DOI：
10.1016/j.febslet.2007.10.022
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发表时间：
2007-11-13
期刊：
影响因子：
3.5
通讯作者：
Ambrogelly, Alexandre
中科院分区：
文献类型：
--
作者：
Namy, Olivier;Zhou, Yu;Ambrogelly, Alexandre
吡咯赖氨酰-tRNA合成酶及其同源抑制因子tRNA（Pyl）介导吡咯赖氨酸（Pyl）在框内UAG密码子处的插入。在抑制位点下游存在称为Pyl插入结构（PYLIS）的RNA发夹结构已显示刺激Pyl在古细菌中的插入。我们在这里研究了在大肠杆菌中使用定量lacZ-luc串联报告系统的PYLIS的存在对Pyl和Pyl类似物N-α-环戊氧基羰基-L-赖氨酸（Cyc）掺入水平的影响。我们表明，PYLIS对Pyl和Cyc掺入水平均无影响。外源地向我们的报告系统提供D-鸟氨酸显著地增加了抑制效率，表明D-鸟氨酸是Pyl的直接前体。(C)2007年欧洲生物化学学会联合会。由Elsevier B出版。版权所有© 2016
Pyrrolysyl-tRNA synthetase and its cognate suppressor tRNA(Pyl) mediate pyrrolysine (Pyl) insertion at in frame UAG codons. The presence of an RNA hairpin structure named Pyl insertion structure (PYLIS) downstream of the suppression site has been shown to stimulate the insertion of Pyl in archaea. We study here the impact of the presence of PYLIS on the level of Pyl and the Pyl analog N-epsilon-cyclopentyloxycarbonyl-L-lysine (Cyc) incorporation using a quantitative lacZ-luc tandem reporter system in an Escherichia coli context. We show that PYLIS has no effect on the level of neither Pyl nor Cyc incorporation. Exogenously supplying our reporter system with D-ornithine significantly increases suppression efficiency, indicating that D-ornithine is a direct precursor to Pyl. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.