Special issue in memory of Robert H. Purdy.
Special issue in memory of Robert H. Purdy.
复制标题
纪念罗伯特·H·珀迪的特刊。
DOI：
10.1007/s00213-014-3585-3
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发表时间：
2014
影响因子：
3.4
通讯作者：
Morrow,ALeslie
中科院分区：
文献类型：
--
作者：
Morrow,ALeslie
为了纪念荣誉。珀迪，我们致力于神经活性类固醇领域的工作，以纪念他.... 2012年9月23日是一个悲伤的日子。斯蒂芬·保罗把这个消息告诉了我，几天之内，鲍勃在科学界的许多合作者和朋友，其中一些人曾经是他的竞争对手，一起分享了他们对他的回忆。这一切都是因为他看到需要一种方法来量化孕酮和脱氧皮质酮（分别为别孕烯醇酮和四氢脱氧皮质酮）的两种主要神经活性代谢物。鲍勃开发了这两种类固醇的抗体，然后发表了通过HPLC分离类固醇的方法，并使用这些抗体通过放射免疫测定法定量适当的等分试样。在他与Steven Paul在NIMH的合作中，他表明压力会增加血浆和大脑中的这些神经活性类固醇，并且大脑中的压力效应先于血浆中的效应。PNAS上的这篇里程碑式的论文开辟了一个全新的研究领域。我很幸运能成为这个项目的博士后，与史蒂夫和鲍勃一起工作。不用说，它重新引导了我的职业生涯，我永远感激鲍勃在那些日子里和这些年里教给我的一切。在他去世后，有一个惊人的情感流露承认他的慷慨与他的类固醇化学的渊博知识。我们领域最伟大的思想家们对鲍勃的许多贡献表示赞赏，他对神经活性类固醇的知识非常广泛和深入，他利用包括质谱法在内的严格方法学方法量化这些分子的开创性努力，他对科学的无休止的奉献，以及他慷慨的友谊。鲍勃·珀迪非常想念。鲍勃在耶鲁大学完成了本科学业，并在哈佛大学获得了化学博士学位。他在类固醇代谢领域工作多年，阐明了儿茶酚雌激素在乳腺癌中的作用。当鲍勃把注意力转向孕酮和脱氧皮质酮的神经活性代谢物时，他已经50多岁了。在发表了他关于抗体特征和压力影响的里程碑式工作之后，他开始与世界各地的研究人员分享抗体。他在早期开发用于人类疾病的神经活性类固醇中发挥了关键作用。他的绵羊多克隆异孕烯醇酮抗体被广泛用于应激，
To honor Robert (Bob) H. Purdy, we dedicate our work in the field of neuroactive steroids to his memory…. September 23, 2012 was a sad day indeed. Stephen Paul delivered the news to me, and within a few days, many of Bob’s collaborators and friends in the scientific community, some of whom had once been his competitors, shared their remembrances of him together.So many of our accomplishments as a field are indebted to Bob. It all started because he saw the need for a way to quantify the two primary neuroactive metabolites of progesterone and deoxycorticosterone (allopregnanolone and tetrahydrodeoxycorticosterone, respectively). Bob developed antibodies to both steroids and then published the methods to separate the steroids by HPLC and quantify the appropriate aliquots by radioimmunoassay using these antibodies. In his work with Steven Paul at the NIMH, he showed that stress elevated these neuroactive steroids in the plasma and brain, and the stress effects in brain preceded effects in plasma. This landmark paper in PNAS opened an entirely new field of research. I was lucky to be the post-doc working with Steve and Bob on this project. Needless to say, it redirected my career and I am forever grateful for all that Bob taught me in those days and over the years. Upon his death, there was an amazing outpouring of sentiment acknowledging his generosity with his vast knowledge of steroid chemistry. The greatest minds in our field expressed their appreciation to Bob for his many contributions, his singularly broad and deep knowledge of neuroactive steroids, his pioneering efforts to quantify these molecules utilizing rigorous methodological approaches that included mass spectrometry, his unending devotion to science, and his generous friendship. Bob Purdy is greatly missed. Bob completed his undergraduate studies at Yale and received his doctorate in chemistry from Harvard. He worked for many years in the field of steroid metabolism and clarified the role of catechol estrogens in breast cancer. Bob was already in his late 50s when he turned his attention to the neuroactive metabolites of progesterone and deoxycorticosterone. After publishing his landmark work on the characterization of the antibodies and the effects of stress, he proceeded to share the antibodies with investigators around the world. He played a key role in the early development of neuroactive steroids for human disease. His sheep polyclonal allopregnanolone antibody became widely used in stress,