Millifluidic droplet analyser for microbiology
Millifluidic droplet analyser for microbiology
复制标题
DOI：
10.1039/c1lc20545e
复制
发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
Bibette, Jerome
中科院分区：
文献类型：
--
作者：
Baraban, Larysa;Bertholle, Fabien;Bibette, Jerome
我们提出了一种新型的微流控液滴分析仪(MDA)，用于精确监测许多(>；10(3))水乳液滴(类似于100 NL)中微生物种群在多代中的动态。作为第一个应用，我们测量细菌菌株的生长速度，并通过在抗生素浓度的细微梯度中培养细菌来确定抗生素头孢噻肟的最小抑菌浓度(MIC)。细胞活性的检测是基于对荧光信号的自动检测，该信号允许监测最大到10(6)个细胞大小的微生物种群。我们相信，该装置有助于研究微生物-环境相互作用的种群动态后果和单个细胞的差异。此外，这台射流机器可能会改进临床测试，因为它简化了对大量微生物种群的筛选，使其自动化和微型化，这些微生物种群在成分微调的隔间中生长和进化。
We present a novel millifluidic droplet analyser (MDA) for precisely monitoring the dynamics of microbial populations over multiple generations in numerous (> 10(3)) aqueous emulsion droplets (similar to 100 nL). As a first application, we measure the growth rate of a bacterial strain and determine the minimal inhibitory concentration (MIC) for the antibiotic cefotaxime by incubating bacteria in a fine gradient of antibiotic concentrations. The detection of cell activity is based on the automated detection of an epifluorescent signal that allows the monitoring of microbial populations up to a size of similar to 10(6) cells. We believe that this device is helpful for the study of population dynamic consequences of microbe-environment interactions and of individual cell differences. Moreover, the fluidic machine may improve clinical tests, as it simplifies, automates and miniaturizes the screening of numerous microbial populations that grow and evolve in compartments with a finely tuned composition.