Keratinocyte growth factor ameliorates dextran sodium sulfate colitis in mice
Keratinocyte growth factor ameliorates dextran sodium sulfate colitis in mice
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DOI：
10.1023/a:1026642715764
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发表时间：
1999-04-01
影响因子：
3.1
通讯作者：
Eysselein, VE
中科院分区：
文献类型：
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作者：
Egger, B;Procaccino, F;Eysselein, VE
角质细胞生长因子（KGF）是结肠粘膜防御和修复的重要介质。本研究的目的是评估和进一步表征外源性KGF管理的影响，利用葡聚糖硫酸钠（DSS）模型的结肠炎小鼠。通过向Balb/c小鼠口服施用DSS（5g/100 mt）持续适当天数来诱导结肠炎。腹腔注射KGF（5 mg/kg？每天一次）或载体（VEH）的治疗（N = 10，每组）。对整个结肠的粘膜损伤进行组织学评估和分级。与对照组（VEH）相比，KGF组的隐窝损伤评分降低了约4倍（分别为2.8 +/- 1.03和11.4 +/- 0.78）。在KGF处理的小鼠中粘膜损伤的显著减少证实了KGF是维持结肠粘膜完整性的关键介质。
Keratinocyte growth factor (KGF) is emerging as an important mediator of mucosal defense and repair in the colon. The aim of the present study was to evaluate and further characterize the effects of exogenous KGF administration utilizing the dextran sodium sulfate (DSS) model of colitis in mice. Colitis was induced via oral administration of DSS (5 g/100 mt) to Balb/c mice for right days. Intraperitoneal administration of KGF (5 mg/kg? once daily) or vehicle (VEH) was initiated 1 hr prior to the induction of the colitis (N = 10, each group). Mucosal injury of the entire colon was histologically assessed and graded. An approximately fourfold reduction in the crypt damage score was noted in the KGF group when compared to controls (VEH) (2.8 +/- 1.03 and 11.4 +/- 0.78, respectively). The significant reduction of mucosal injury in KGF treated mice confirms that KGF is a key mediator maintaining the integrity of the colonic mucosa.