Raman spectroscopic imaging of pH values in cancerous tissue by using polyaniline@gold nanoparticles
Raman spectroscopic imaging of pH values in cancerous tissue by using polyaniline@gold nanoparticles
复制标题
使用聚苯胺@金纳米粒子对癌组织中的 pH 值进行拉曼光谱成像
DOI：
10.1007/s00604-019-3265-4
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发表时间：
2019-03-01
影响因子：
5.7
通讯作者：
Hu, Yuling
中科院分区：
文献类型：
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作者：
Li, Zicheng;Xia, Ling;Hu, Yuling
由聚苯胺和金纳米粒子（PANI@AuNPs）组成的核壳纳米复合材料被证明能够通过表面增强拉曼散射（SERS）光谱来监测细胞内的pH值。该方法利用PANI的pH响应特性和AuNPs的SERS增强效应。当pH值从4.6增加到7.4时，PANI拉曼峰在1164 cm-1处的强度降低。这是正常细胞和癌细胞中遇到的pH值范围。将PANI@AuNPs掺入HeLa癌细胞和5种其他类型的细胞中，用于基于拉曼的pH值成像。结果表明，这种pH纳米探针可用于正常细胞和癌细胞的成像。核壳复合材料也被应用于组织成像。在我们看来，这种核-壳纳米探针是成像癌组织pH值的有价值的工具。
图形摘要示意图的核壳纳米复合材料，聚苯胺@金纳米粒子，这是通过一种快速的方法合成。随着溶液pH值由碱性变为酸性，聚苯胺由蓝壳的翠绿亚胺基（EB）转变为绿壳的翠绿亚胺盐（ES，绿色壳）。由于聚苯胺的pH响应特性与金纳米粒子的表面增强拉曼散射效应相结合。聚苯胺@金纳米粒子成功地应用于细胞内pH探针。
AbstractA core-shell nanocomposite consisting of polyaniline and gold nanoparticles (PANI@AuNPs) is shown to enable intracellular monitoring of pH values by surface-enhanced Raman scattering (SERS) spectroscopy. The method exploits the pH-responsive property of PANI and the SERS-enhancing effect of AuNPs. The intensity of the PANI Raman peak at 1164 cm−1 decreases on increasing the pH value from 4.6 to 7.4. This is the pH range encountered in normal cells and in cancer cells. The PANI@AuNPs were incorporated into HeLa cancer cells and 5 other kinds of cells for Raman based imaging of pH values. The results show that this pH nanoprobe can be applied for imaging of both normal cells and cancer cells. The core-shell composite was also applied to tissue imaging. In our perception, this core-shell nanoprobe is a valuable tool for imaging pH values of cancerous tissue.
Graphical abstractSchematic presentation of a core-shell nanocomposite, polyaniline@gold nanoparticle, which was synthesized via a rapid method. With the pH of solution changing from alkaline to acidic, the polyaniline can change from emeraldine base (EB, blue shell) transition to emeraldine salt (ES, green shell) transition. Due to the pH-responsive property of polyaniline combined with the surface-enhanced Raman scattering spectroscopy effect of AuNPs. The polyaniline@gold nanoparticles were successfully applied as an intracellular pH probe.