PARTIAL BETA -TUBULIN GENE SEQUENCES FOR EVOLUTIONARY STUDIES IN THE BASIDIOMYCOTINA
PARTIAL BETA -TUBULIN GENE SEQUENCES FOR EVOLUTIONARY STUDIES IN THE BASIDIOMYCOTINA
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用于担子菌亚门进化研究的部分β-微管蛋白基因序列
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
D. Royse
中科院分区：
文献类型：
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作者：
M. Thon;D. Royse
用4对引物扩增了担子菌亚门3-微管蛋白基因。从香菇（Lentinula spp.）分离物中扩增出一个约500 bp的片段，包含2个内含子和133个密码子，拟青霉属，和几个相关的分类群。内含子区在侧耳属中高度多态。以及侧耳属和香菇属之间的关系。两个湖砖红壤分离物之间的相似性为99%，与侧耳属分离物的相似性为80.7%~ 82.8%。在蛋白质编码区也存在足够的多态性，以区分不同种的Plepherus。虎斑香菇和灵芝具有独特的进化关系。系统发育分析为子囊菌基因间存在古老的基因重复提供了证据。在基因复制的分歧之后，担子菌谱系附着在构巢曲霉BenA/禾生炭疽菌Tub 2簇附近的点处。现有的真菌之间的变异水平表明，该基因将是有用的，在更广泛的真菌群体之间的高阶关系的系统发育研究。
Four primer pairs comprising six individ- ual primers were used to amplify regions of 3-tubulin genes from members of the Basidiomycotina. A re- gion of approximately 500 bp, spanning two introns and 133 codons, was PCR-amplified and sequenced from isolates of Lentinula spp., Pleurotus spp., and several related taxa. Intronic regions were highly polymorphic among Pleurotus spp. and between Pleu- rotus and Lentinula. Two L. lateritia isolates were 99% similar to each other and 80.7%-82.8% similar to Pleurotus species. Sufficient polymorphism also ex- isted within protein coding regions to differentiate among species of Pleurotus. Lentinus tigrinus and Ganoderma lucidum shared a unique evolutionary lin- eage. The phylogenetic analysis provided evidence for an ancient gene duplication among ascomycete genes. The basidiomycete lineage is attached at a point near the Aspergillus nidulans BenA/Colletotri- chum graminicola Tub2 cluster, after the divergence of the gene duplication. The existing level of varia- tion among the fungi examined indicates that this gene will be useful in phylogenetic investigations of higher order relationships among broader groups of fungi.