Drosophila Eph receptor guides specific axon branches of mushroom body neurons
Drosophila Eph receptor guides specific axon branches of mushroom body neurons
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DOI：
10.1242/dev.02353
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发表时间：
2006-05-01
期刊：
影响因子：
4.6
通讯作者：
Thomas, JB
中科院分区：
文献类型：
--
作者：
Boyle, M;Nighorn, A;Thomas, JB
保守的Eph受体及其Ephrin配体调节许多发育过程，包括轴突导向。与大型脊椎动物Eph/Ephrin家族相比，果蝇具有单个Eph受体和单个Ephrin配体，两者都在发育中的神经系统内表达。在这里，我们表明，Eph和Ephrin可以作为一个功能性的受体-配体对在体内。令人惊讶的是，与先前使用RNA干扰技术的结果相反，完全缺乏Eph功能的胚胎没有表现出明显的轴突导向缺陷。然而，Eph/Ephrin信号是蘑菇体正常发育所必需的。在野生型中，蘑菇体神经元分叉并向不同的靶区域延伸不同的分支。在Eph突变体中，这些神经元正常分叉，但在许多情况下，背侧分支未能投射到其适当的靶区域。因此，Eph/Ephrin信号传导作用于引导蘑菇体分支的子集到它们正确的突触靶点。
The conserved Eph receptors and their Ephrin ligands regulate a number of developmental processes, including axon guidance. In contrast to the large vertebrate Eph/Ephrin family, Drosophila has a single Eph receptor and a single Ephrin ligand, both of which are expressed within the developing nervous system. Here, we show that Eph and Ephrin can act as a functional receptor-ligand pair in vivo. Surprisingly, and in contrast to previous results using RNA-interference techniques, embryos completely lacking Eph function show no obvious axon guidance defects. However, Eph/Ephrin signaling is required for proper development of the mushroom body. In wild type, mushroom body neurons bifurcate and extend distinct branches to different target areas. In Eph mutants, these neurons bifurcate normally, but in many cases the dorsal branch fails to project to its appropriate target area. Thus, Eph/Ephrin signaling acts to guide a subset of mushroom body branches to their correct synaptic targets.