Comparison of the distributions of D1 and D2 dopamine receptor mRNAs in rat brain.-猫眼课题宝

DOI：

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发表时间：

1991-12

期刊：

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology

影响因子：

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通讯作者：

中科院分区：

其他

文献类型：

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作者：

J. Meador-Woodruff;A. Mansour;Daniel J. Healy;Rebekah Kuehn;Qun-Yong Zhou;J. Bunzow;Huda Akil;Olivier Civelli;Stanley J. Watson

用原位杂交法测定了编码多巴胺D1和D2受体的信使RNA在大鼠脑内的分布。高水平的两种mRNA被发现在传统的多巴胺感受性区域的大脑，包括尾壳核，丘脑核，和嗅结节;低水平的两个被发现在一些其他的神经结构，如外侧隔，嗅球，下丘脑，和皮质。高水平的D2，但不是D1受体mRNA被确定在中脑多巴胺细胞组，这表明在黑质和腹侧被盖区发现的自体受体是专门的D2。其他显示这两种mRNA差异分布的区域包括垂体、杏仁核和海马。定量密度分析显示，在研究的大多数大脑区域中，这两种信息都存在，D1和D2受体mRNA的数量大致相等。最后，通过尾壳核使用薄（2.5微米）切片，发现大约一半的细胞对D1受体mRNA呈阳性，大约75%的细胞含有D2受体mRNA。随后的连续切片分析显示，D1和D2受体mRNA共定位发生在33% +/- 7%的尾壳核细胞中：约一半含有D1受体mRNA的细胞也含有D2受体mRNA，约一半的D2受体mRNA阳性细胞也含有D1受体mRNA。这些结果表明，D1和D2多巴胺感受细胞之间有相当大的重叠，并提供了一个基础，为未来的监管研究多巴胺系统在大脑中的一个定义的解剖背景。

The distributions of messenger RNAs encoding both the D1 and D2 dopamine receptors have been determined in the rat brain by in situ hybridization. High levels of both mRNAs were found in the traditional dopaminoceptive regions of brain, including the caudate-putamen, nucleus accumbens, and olfactory tubercle; lower levels of both were found in a number of other neural structures, such as the lateral septum, olfactory bulb, hypothalamus, and cortex. High levels of D2 but not D1 receptor mRNA were identified in the midbrain dopamine cell groups, suggesting that the autoreceptors found in the substantia nigra and ventral tegmental area are exclusively D2. Other areas demonstrating differential distribution of these two mRNAs included the pituitary, amygdala, and hippocampus. Quantitative densitometric analysis revealed that in most of the brain regions studied in which both messages exist, the amounts of D1 and D2 receptor mRNAs were approximately equal. Finally, using thin (2.5-micron) sections through the caudate-putamen, about half of all cells were found to be positive for D1 receptor mRNA, and approximately 75% of cells contained D2 receptor mRNA. Subsequent analysis in sequential sections revealed that co-localization of D1 and D2 receptor mRNA occurred in 33% +/- 7% of all caudate-putamen cells: about half of all cells containing D1 receptor mRNA also contained D2 receptor mRNA, and approximately half of all D2 receptor mRNA-positive cells also contained D1 receptor mRNA. These results indicate that there is considerable overlap between D1 and D2 dopaminoceptive cells, and provide a basis for future regulatory studies of dopamine systems in brain within a defined anatomic context.