Effectiveness of exercise-induced cytokines in alleviating arthritis symptoms in arthritis model mice
Effectiveness of exercise-induced cytokines in alleviating arthritis symptoms in arthritis model mice
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DOI：
10.2535/ofaj.93.81
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发表时间：
2016-11-01
期刊：
影响因子：
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通讯作者：
Yamada, Kouji
中科院分区：
文献类型：
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作者：
Kito, Takumi;Teranishi, Toshio;Yamada, Kouji
最近，日本的健康意识不断增强，现在建议积极锻炼以预防与生活方式有关的疾病。细胞因子的活动在维持身体许多器官的健康方面有许多积极的作用。肌因子是骨骼肌在运动刺激下分泌的细胞因子，近年来引起了人们的广泛关注。在日本，大约有70万患者患有类风湿性关节炎，使其成为最普遍的自身免疫性疾病，需要积极预防和治疗。本研究以自发性关节炎（SKG/Jcl）小鼠模型为研究对象，从发病前开始进行持续运动刺激，观察抗炎和炎性细胞因子分泌对关节炎的影响。对于这种刺激，我们开发了一种结合震动和振动的装置。结果显示，运动刺激延缓了关节炎的发作，减缓了它的发展。关节软骨增厚，软骨细胞多发聚集。此外，运动刺激增加了IL-6、IL-10和IL-15的表达，抑制了tnf - α的表达。从这些结果，我们推断IL-6和IL-10的抗炎作用，在运动刺激下表达增加，抑制tnf - α的炎症活性，可能延迟关节炎的发生并减缓其进展。在这些发现的基础上，可以开发预防和治疗关节炎的新方法。
Recently, health awareness in Japan has been increasing and active exercise is now recommended to prevent lifestyle-related diseases. Cytokine activities have many positive effects in maintaining the health of a number of organs in the body. Myokines are cytokines secreted by skeletal muscles in response to exercise stimulation, and have recently generated much attention. Around 700,000 patients in Japan suffer from rheumatoid arthritis, making it the most prevalent autoimmune disease that requires active prevention and treatment. In the present study, a mouse model of spontaneous arthritis (SKG/Jcl) was subjected to continuous exercise stimulation, starting before the disease onset, to examine the effects of anti-inflammatory and inflammatory cytokine secretion on arthritis. For this stimulation, we developed a device that combines shaking and vibration. The results revealed that exercise stimulation delayed the onset of arthritis and slowed its progression. Thickened articular cartilage and multiple aggregates of chondrocytes were also observed. Further, exercise stimulation increased the expression of IL-6, IL-10, and IL-15, and inhibited TNF-alpha expression. From these results, we infer that the anti-inflammatory effects of IL-6 and IL-10, which showed increased expression upon exercise stimulation, inhibited the inflammatory activity of TNF-alpha and possibly delayed the onset of arthritis and slowed its progression. Novel methods for preventing and treating arthritis under clinical settings can be developed on the basis of these findings.