Induction of functional prolactin receptors by follicle-stimulating hormone in rat granulosa cells in vivo and in vitro.
Induction of functional prolactin receptors by follicle-stimulating hormone in rat granulosa cells in vivo and in vitro.
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体内和体外大鼠颗粒细胞中促卵泡激素诱导功能性催乳素受体。
DOI：
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发表时间：
1979
影响因子：
4.8
通讯作者：
G. Erickson
中科院分区：
文献类型：
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作者：
C. Wang;A. Hsueh;G. Erickson
研究了卵泡刺激素（FSH）对颗粒细胞催乳素（PRL）受体的调节作用。在化学成分确定的培养基中孵育2天后，lz 51- PRL与培养的颗粒细胞的特异性结合很低（1.01 ± 0.23 fmol/106细胞）。与此相反，FSH（100 ng/ ml）的加入导致lz 51-PRL的特异性结合增加3倍（3.86 fmol/106细胞）。在体内注射FSH 2天后，颗粒细胞的12 ′ I-PRL也同样增加3倍。FSH（1 pg）和人绒毛膜促性腺激素（hCG; 1 pg）不能取代12 'I-PRL，但人生长激素（hGH）在竞争12' I-PRL结合位点方面与PRL一样有效。研究了PRL对孕酮、20 α-二氢孕酮和雌激素生物合成的影响。在含有100 nM雄烯二酮（芳香化酶底物）的确定培养基中孵育B天期间，来自未引发动物的对照和PRL处理的细胞分泌可忽略不计的类固醇激素。然而，在体外用FSH（100 μ g/ml）预处理2天后，这些细胞对PRL（1 μ g/ml）的反应是孕酮和20 α-二氢孕酮的产量分别增加5倍和3倍。相比之下，PRL并没有增加这些细胞的雌激素形成，这表明PRL的作用是专门耦合到孕激素合成。催乳素对孕酮合成的刺激作用与剂量有关，催乳素的最小和表观最大有效剂量分别为30 μ g/ml和1 μ g/ml。20 α-二氢孕酮在PRL中也有剂量相关的增加，但低于孕酮的反应。在体内用FSH预处理2天的颗粒细胞中观察到对PRL的类固醇生成反应相当，表明我们的体外观察反映了正常的生理事件。与对照组和FSH处理的细胞相比，PRL显著降低了孕酮/20 α-二氢孕酮比值（a倍），表明PRL降低了20 α-羟基类固醇脱氢酶活性。这些结果表明：1）FSH在体外和体内刺激颗粒细胞特异性PRL受体的形成，2）FSH诱导的PRL受体
The role of follicle-stimulating hormone (FSH) in the regulation of prolactin (PRL) receptors in granulosa cells was investigated. After 2 days of incubation in a chemically defined medium, the specific binding of lz51- PRL to cultured granulosa cells was low (1.01 + 0.23 fmol/106 cells). In contrast, addition of FSH (100 ng/ ml) caused a 3-fold increase in the specific binding of lz51-PRL (3.86 f 0.33 fmol/106 cells). In viuo administra- tion of FSH for 2 days caused a similar 3-fold increase in 12’I-PRL to granulosa cells. FSH (1 pg) and human chorionic gonadotropin (hCG; 1 pg) did not displace 12’I-PRL, but human growth hormone (hGH) was as effective as PRL in competing for 12’I-PRL binding sites. The effect of PRL on progesterone, 20a-dihydropro- gesterone, and estrogen biosynthesis was investigated. During a B-day incubation in defined media containing 100 nM androstenedione (aromatase substrate), control and PRL-treated cells from unprimed animals secreted negligible amounts of steroid hormones. However, after pretreatment for 2 days in vitro with FSH (100 rig/ml), these cells responded to PRL (1 gg/ml) by showing a 5- fold and 3-fold increase in progesterone and 2Oa-dihy- droprogesterone production, respectively. In contrast, PRL did not increase estrogen formation by such cells, indicating the PRL effect was specifically coupled to progestin synthesis. The stimulatory effect of PRL on progesterone synthesis was dose-related; the minimal and apparent maximal effective doses of PRL were 30 rig/ml and 1 pg/ml, respectively. A dose-related in- crease in 20a-dihydroprogesterone was also observed with PRL but it was less than the progesterone re- sponse. Comparable steroidogenic responses to PRL were observed with granulosa cells pretreated with FSH for 2 days in viva, suggesting our in vitro obser- vations reflect a normal physiological event. The pro- gesterone/20a-dihydroprogesterone ratio was signifi- cantly decreased (a-fold) by PRL over control and FSH- treated cells, suggesting PRL decreased 20a-hydroxy- steroid dehydrogenase activity. These results demonstrate that 1) FSH stimulates the formation of specific PRL receptors in granulosa cells in vitro and in uiuo, 2) the FSH-induced PRL receptors