Characterization of the agr2 gene, a homologue of X-laevis anterior gradient 2, from the zebrafish, Danio rerio
Characterization of the agr2 gene, a homologue of X-laevis anterior gradient 2, from the zebrafish, Danio rerio
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DOI：
10.1016/j.modgep.2006.11.003
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发表时间：
2007-02-01
影响因子：
1.2
通讯作者：
Hwang, Sheng-Ping L.
中科院分区：
文献类型：
--
作者：
Shih, Li-Jane;Lu, Yu-Fen;Hwang, Sheng-Ping L.
我们鉴定了斑马鱼（Danio rerio）前梯度2同源基因（agr2）。Agr2包含一个513 bp的开放阅读框，编码171个氨基酸。推断的氨基酸序列比较表明，斑马鱼agr2蛋白与人类、小鼠、河鲀和大西洋鲑鱼的前梯度2同源基因（HAGR2、MAgr2、Tagr2和Sagr2）的氨基酸序列相似性较高（80-89%），而与非洲爪蟾、小鼠和人类的前梯度2同源基因（XAG-2、XAG-1、XAgr2、MAgr3和HAGR3）的序列相似性较低（67-71%）。系统发育和合成分析表明，斑马鱼的agr2基因与人类的agr2基因和小鼠的agr2基因同源。全安装原位杂交表明，斑马鱼的表皮、嗅球、耳小泡、咽、食道、气管、膀胱、肠等大部分含有粘液分泌细胞的器官中都有agr2的表达。此外，半定量RT-PCR显示，agr2在成鱼的鳃、咽/食管、鱼鳔/气动管和肠道中表达。相比之下，非洲爪蟾前梯度2同源基因主要表达于外胚层器官，包括骨水泥腺和耳小泡，而人和小鼠前梯度2同源基因主要分布于气管、肺、胃、肠和结肠等内胚层器官。(c) 2006 Elsevier B.V.版权所有
We characterized a zebrafish (Danio rerio) anterior gradient 2 homologue (agr2) gene. agr2 contains an open reading frame of 513 bp encoding 171 amino acids. Deduced amino acid sequence comparison showed that the zebrafish agr2 protein shares high (80-89%) amino acid sequence similarity with those homologues of anterior gradient 2 (HAGR2, MAgr2, Tagr2, and Sagr2) from the human, mouse, pufferfish, and Atlantic salmon, while sharing less (67-71%) sequence similarity with those anterior gradient 2 genes (XAG-2, XAG-1, XAgr2, MAgr3, and HAGR3) from Xenopus laevis, mouse, and human. Both phylogenetic and syntenic analyses indicate that zebrafish agr2 is the orthologue of Human A GR2 and mouse Agr2 genes. Whole-mount in situ hybridization indicated that zebrafish agr2 is expressed in most organs, such as epidermis, olfactory bulbs, otic vesicles, pharynx, esophagus, pneumatic duct, swim bladder, and intestine, which contain mucus-secreting cells. Moreover, semi-quantitative RT-PCR demonstrated agr2 is expressed in the gill, pharynx/esophagus, swim bladder/pneumatic duct, and intestine in the adult fish. In contrast, Xenopus anterior gradient 2 homologues are mainly expressed in ectoderm-derived organs including the cement gland and otic vesicles, while human and mouse anterior gradient 2 orthologues are mainly distributed in endoderm-derived organs including the trachea, lungs, stomach, intestines, and colon. (c) 2006 Elsevier B.V. All rights reserved.