Chemical characterization of red wine grape (Vitis vinifera and Vitis interspecific hybrids) and pomace phenolic extracts and their biological activity against Streptococcus mutans.
Chemical characterization of red wine grape (Vitis vinifera and Vitis interspecific hybrids) and pomace phenolic extracts and their biological activity against Streptococcus mutans.
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红酿酒葡萄（葡萄和种间杂交种）和果渣酚类提取物的化学特性及其对变形链球菌的生物活性。
DOI：
10.1021/jf0722405
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发表时间：
2007
影响因子：
6.1
通讯作者：
H. Koo
中科院分区：
文献类型：
--
作者：
Joanne Thimothe;Illeme A Bonsi;O. Padilla;H. Koo
葡萄富含潜在的生物活性多酚。然而，酚含量是可变的，取决于葡萄品种，并可能在酿造过程中进行修改。在这项研究中，我们研究了从几种红葡萄酒葡萄品种及其酿酒副产物（果渣）中提取的酚类提取物的化学成分和生物活性对变形链球菌（一种众所周知的牙齿病原体）的一些毒力特性的影响。从葡萄品种品丽珠和黑皮诺以及葡萄种间杂交品种黑巴科和黑莱特中提取葡萄酚类提取物。花青素和黄烷-3-醇含量因葡萄品种和提取物类型（全果与发酵果渣）的不同而有很大差异。在62.5 μ g/mL浓度下，所有葡萄酚提取物均显著抑制葡萄糖基转移酶B和C (70-85%) （P < 0.01）。葡萄提取物可抑制变形链球菌细胞糖酵解ph下降，但不影响细菌活力；该效应可归因于部分抑制f - atp酶活性（在125微克/毫升时抑制30-65%；P < 0.01）。发酵渣的生物活性与全果葡萄提取物相当或显著优于全果葡萄提取物。结果表明，葡萄酚类提取物，尤其是果渣提取物，尽管其酚类含量存在很大差异，但对突变链球菌的特定毒力性状都有很好的抑制作用。
Grapes are rich sources of potentially bioactive polyphenols. However, the phenolic content is variable depending on grape variety, and may be modified during vinification. In this study, we examined the chemical composition and biological activity of phenolic extracts prepared from several red wine grape varieties and their fermented byproduct of winemaking (pomace) on some of the virulence properties of Streptococcus mutans a well-known dental pathogen. Grape phenolic extracts were obtained from Vitis vinifera varieties Cabernet Franc and Pinot Noir and Vitis interspecific hybrid varieties Baco Noir and Noiret. The anthocyanins and flavan-3-ols content were highly variable depending on grape variety and type of extract (whole fruit vs fermented pomace). Nevertheless, all grape phenolic extracts remarkably inhibited glucosyltransferases B and C (70-85% inhibition) at concentrations as low as 62.5 microg/mL (P < 0.01). Furthermore, the glycolytic pH-drop by S. mutans cells was inhibited by the grape extracts without affecting the bacterial viability; an effect that can be attributed to partial inhibition of F-ATPase activity (30-65% inhibition at 125 microg/mL; P < 0.01). The biological activity of fermented pomace was either as effective as or significantly better than whole fruit grape extracts. The results showed that grape phenolic extracts, especially from pomace, are highly effective against specific virulence traits of S. mutans despite major differences in their phenolic content.