Keratin films for ocular surface reconstruction: Evaluation of biocompatibility in an in-vivo model
Keratin films for ocular surface reconstruction: Evaluation of biocompatibility in an in-vivo model
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DOI：
10.1016/j.biomaterials.2014.11.038
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发表时间：
2015-02-01
期刊：
影响因子：
14
通讯作者：
Schrader, Stefan
中科院分区：
文献类型：
--
作者：
Borrelli, Maria;Joepen, Nadine;Schrader, Stefan
羊膜移植是目前眼表重建的标准方法，但近年来角蛋白膜被提出作为替代材料。本研究的目的是在兔模型中评价KF的角膜生物相容性。对46只新西兰白色家兔进行角膜基质内囊袋的解剖，其中插入AM或KF植入物，并观察10天和4周。一半的动物接受局部类固醇，而另一半则没有。在随访结束时，进行临床和组织学检查，以评价透明度、炎症和降解。10天后，接受和不接受类固醇治疗的KF植入眼的临床和组织学结果似乎相当。4周后，在所有KF植入眼中观察到相当的临床结果，而与类固醇治疗眼相比，非类固醇的炎症评分较低，沿着角蛋白膜的降解速率较高。因此，人发角蛋白膜在体内显示出良好的生物相容性和透明性。局部类固醇的施用似乎减缓了植入物降解，这可能对组织整合和基质再生的调节很重要。(C)2014爱思唯尔有限公司版权所有。
Amniotic membrane (AM) transplantation is the clinical standard for ocular surface reconstruction, however recently keratin film (KF) has been proposed as an alternative material. Aim of the current study was to evaluate corneal biocompatibility of KF in a rabbit model. Forty-six New Zealand white rabbits underwent dissection of a corneal intrastromal pocket in which an AM or KF implant was inserted and observed for 10 days and for 4 weeks. Half of animals received topical steroids, while the other half were left without. At the end of the follow-up clinical and histology examinations were performed to evaluate transparency, inflammation and degradation. After 10 days the clinical and the histology results appeared to be comparable in KF implanted eyes treated with and without steroids. After 4 weeks, comparable clinical results were observed in all KF implanted eyes, while the inflammation score was lower in non-steroid compared to steroid treated eyes along with a higher degradation rate of the keratin films. In conclusion, keratin films from human hair show a good biocompatibility and transparency in vivo. The administration of topical steroids seems to slow down implant degradation which might be important for the modulation of tissue integration and matrix regeneration. (C) 2014 Elsevier Ltd. All rights reserved.