Alterations of gut microbiota in patients with active pulmonary tuberculosis in China: a pilot study
Alterations of gut microbiota in patients with active pulmonary tuberculosis in China: a pilot study
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中国活动性肺结核患者肠道微生物群的变化：一项试点研究
DOI：
10.1016/j.ijid.2021.08.064
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发表时间：
2021
影响因子：
8.4
通讯作者：
Biao Xu
中科院分区：
文献类型：
--
作者：
Wenpei Shi;Yi Hu;Zhu Ning;Fan Xia;Meiying Wu;Yue O.O. Hu;Cheng Chen;Stefanie Prast-Nielsen;Biao Xu
背景：本研究的目的是确定结核病 (TB) 患者和健康对照 (HC) 之间肠道微生物群多样性、组成和功能的差异。方法：在中国三个城市进行了一项横断面研究。通过 16S rRNA 基因测序分析了 94 名未接受治疗的结核病患者和 62 名健康对照者的粪便样本。根据结核病诊断前是否使用非特异性抗生素，将结核病患者进一步分为未使用抗生素和抗生素暴露。结果：与健康对照相比，未使用抗生素的结核病患者肠道微生物群落不同（P < 0.005），香农多样性降低（P < 0.005）。在结核病患者中，产生短链脂肪酸（SCFA）的属（例如Lachnospiraceae ND3007组（log2（FC）= -2.74））的相对丰度较低，而一些条件病原体相关属（例如肠球菌（log2（FC）= 12.05）和Rothia（log2（FC）= 6.322））的相对丰度较高。此外，41%的患者在结核病诊断前接受了抗生素治疗。抗生素暴露与 α 多样性的额外减少和 SCFA 产生细菌的消耗相关。微生物功能分析显示，与健康对照相比，结核病患者的氨基酸和脂肪酸生物合成能力较低。结论：观察到结核病患者肠道微生物群组成和代谢途径的显着变化。抗生素暴露可能会改变结核病患者的肠道微生物群，在抗结核治疗中应考虑到这一点。
Background: The aim of this study was to identify the differences in diversity, composition, and function of the gut microbiota between tuberculosis (TB) patients and healthy controls (HCs)..Methods: A cross-sectional study was conducted in three cities of China. Stool samples from 94 treatment-naive TB patients and 62 HCs were analyzed by 16S rRNA gene sequencing. TB patients were further divided into antibiotic-free and antibiotic-exposure according to their use of non-specific antibiotics before the TB diagnosis..Results: Compared with HCs, antibiotic-free TB patients presented a different gut microbial community (P < 0.005) and decreased Shannon diversity (P < 0.005). Among TB patients, the relative abundances of short-chain fatty acid (SCFA)-producing genera such as Lachnospiraceae ND3007 group (log2(FC) = −2.74) were lower, while several conditional pathogen-related genera such as Enterococcus (log2(FC) = 12.05) and Rothia (log2(FC) = 6.322) were at higher levels. In addition, 41% of patients received antibiotics before TB diagnosis. Antibiotic exposure was correlated with an additional reduction in α diversity and depletion of SCFA-producing bacteria. Microbial functional analysis revealed that the biosynthesis capacity of amino acids and fatty acids was lower among TB patients compared to HCs..Conclusions: Significant alterations in gut microbiota composition and metabolic pathways of TB patients were observed. Antibiotic exposure could alter the gut microbiota of TB patients, which should be considered in anti-TB treatment.