Time course of inflammatory cytokines in acute ischemic stroke patients and their relation to inter-alfa trypsin inhibitor heavy chain 4 and outcome.
Time course of inflammatory cytokines in acute ischemic stroke patients and their relation to inter-alfa trypsin inhibitor heavy chain 4 and outcome.
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DOI：
10.4103/0972-2327.99707
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发表时间：
2012-07
影响因子：
1.7
通讯作者：
Daginawala HF
中科院分区：
文献类型：
--
作者：
Nayak AR;Kashyap RS;Kabra D;Purohit HJ;Taori GM;Daginawala HF
脑卒中预后的生物标志物是急性缺血性脑卒中（AIS）患者治疗的迫切需要。目的：评价AIS患者炎症因子的变化过程及其与间α -胰蛋白酶抑制剂重链4 （ITIH4）的比较及AIS后的预后。在AIS患者入院、24小时、48小时、72小时、144小时和出院时收集的一系列血液样本中估计了12种炎症细胞因子和ITIH4 （n = 5）。12种细胞因子中，只有白细胞介素(IL)-2、肿瘤坏死因子-α (TNF-α)、IL-10、IL-6、IL- 1b和IL-8在试剂盒的可测范围内（10 pg/mL）。入院时患者IL-2水平较高，随访时IL-2水平下降（P < 0.05）。TNF-α在24 h开始升高（P < 0.05）， 72 h后逐渐降低（P < 0.05）， IL-10在72 h开始下降（P < 0.05）， 144 h后逐渐升高（P < 0.05）。同样，ITIH4在72 h早期下调，随着病情的好转进一步升高。ITIH4与IL-10和计算机断层扫描梗死体积相关。AIS患者血清IL-6、IL-1B、IL-8均升高，但无明显规律。连续测定IL-10、IL-2、TNF-α和ITIH4可能有助于AIS后临床预后的随访。
Biomarker for prognosis of stroke is urgently needed for the management of acute ischemic stroke (AIS) patients. To evaluate the course of inflammatory cytokines in AIS patients and its comparison with inter-alfa trypsin inhibitor heavy chain 4 (ITIH4) and outcome after AIS. A panel of 12 inflammatory cytokines and ITIH4 were estimated in serial blood samples collected at admission, 24 h, 48 h, 72 h, 144 h and at discharge of AIS patients (n = 5). Out of the 12 cytokines, only interleukin (IL)-2, tumor necrosis factor-alfa (TNF-α), IL-10, IL-6, IL-1B and IL-8 were in the measurable range of the kit (10 pg/mL). We found high IL-2 at admission, which decreased (P < 0.05) in the follow-up samples. TNF-α initially increases (P < 0.05) at 24 h followed by gradual decrease (P < 0.05) after 72 h. IL-10 decreases initially (P < 0.05) till 72 h as compared with its level at admission and then increases (P < 0.05) after 144 h. Similarly, ITIH4 was down-regulated in the early 72 h followed by further increase with improvement of the patient. ITIH4 correlates with IL-10 and computed tomography scan infarct volume. Serum IL-6, IL-1B and IL-8 increased in the AIS patients, but did not show any pattern. Serial measurement of IL-10, IL-2 and TNF-α and ITIH4 may be useful for the follow-up of clinical outcome after AIS.