Up-regulation of Wip1 involves in neuroinflammation of retinal astrocytes after optic nerve crush via NF-kappa B signaling pathway
Up-regulation of Wip1 involves in neuroinflammation of retinal astrocytes after optic nerve crush via NF-kappa B signaling pathway
复制标题
Wip1上调通过NF-kappa B信号通路参与视神经挤压后视网膜星形胶质细胞的神经炎症
DOI：
10.1007/s00011-016-0952-z
复制
发表时间：
2016
影响因子：
6.7
通讯作者：
Li Min
中科院分区：
文献类型：
--
作者：
Zhong Haibin;Cui Ling;Xu Fan;Chen Lifei;Jiang Li;Huang Hui;Xu Jiping;Zhao Xin;Li Li;Zeng Siming;Li Min
ObjectiveTo evaluate the expression and possible roles of Wip1 in retinal astrocytes after optic nerve crush (ONC).MethodsExpressions of Wip1, GFAP, and p-p65 in ONC model were analyzed by Western blot and immunofluorescence. The mRNA expressions of the pro-inflammatory cytokines (IL-8, TNF-α, IL-6 and IL-1β) were analyzed by RT-PCR.ResultsWip1 was up-regulated at 14 days after ONC by Western blot and immunofluorescence. The changes of Wip1 were striking in astrocytes. Furthermore, the protein expression level of p-p65 was paralleled with Wip1 in a time-dependent manner by ONC. In addition, co-localization of Wip1 with Phospho-NF-κB-p65 (p-p65) was detected. Finally, the mRNA expressions of the pro-inflammatory cytokines (IL-8, TNF-α, IL-6 and IL-1β) were significantly increased in retina after ONC.ConclusionsThese data were consistent with the hypothesis that Wip1 was implicated in neuroinflammation of retinal astrocytes after ONC via NF-κB signaling pathway.
