BARBITURATES AND OXIDATIVE PHOSPHORYLATION
BARBITURATES AND OXIDATIVE PHOSPHORYLATION
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DOI：
10.1042/bj0760047
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发表时间：
1960-01-01
影响因子：
4.1
通讯作者：
PARKER, VH
中科院分区：
文献类型：
--
作者：
ALDRIDGE, WN;PARKER, VH
氧巴比妥类药物苯巴比妥、阿米妥（阿莫巴比妥）和六巴比妥抑制但不解偶联以丙酮酸为底物的肝线粒体氧化磷酸化。硫代巴比妥类药物硫喷妥、贝妥纳尔（5-烯丙基-5-异丁基-硫代巴比妥钠）和凯米沙尔（硫巴比妥）抑制并在一定程度上解偶联氧化磷酸化。这种解偶联似乎与它们激活线粒体腺苷三磷酸酶的能力有关。脑线粒体的结果与肝线粒体的结果在定性上一致，但由于制备的不稳定性，这些结果的解释是不确定的。氧巴比妥酸盐抑制2,4-二硝基苯酚刺激的氧摄取。而2,4-二硝基苯酚刺激的腺苷三磷酸酶活性不受抑制。硫代巴比妥类药物抑制氧摄取，并在较小程度上抑制腺苷三磷酸酶。在琥珀酸盐存在的情况下，阿米妥和凯密塔尔都不能抑制氧化。根据目前对呼吸链的看法，对这些结果进行了解释。
The oxybarbiturates pheno-barbital, Amytal (amobarbital) and hexobarbital inhibit but do not uncouple oxidative phosphorylation of liver mitochondria with pyruvate as substrate. The thiobarbiturates thiopental, Baytenal (sodium 5-allyl-5-isobutyl-thiobarbiturate) and Kemithal (thialbarbital) inhibit and, to a certain extent, uncouple oxidative phosphorylation. The uncoupling appears to be correlated with their ability to activate mitochondrial adenosine triphosphatase. Results with brain mitochondria are in qualitative agreement with those with liver mitochondria but owing to the instability of the preparation the interpretation of these results is uncertain. The oxybarbiturates inhibited oxygen uptake stimulated by 2,4-dinitrophenol. In contrast adenosine triphosphatase activity stimulated by 2,4-dinitrophenol was not inhibited. The thiobarbiturates inhibit the oxygen uptake and, to a lesser extent, the adenosine triphosphatase. Neither Amytal nor Kemithal inhibits oxidation in the presence of succinate. The interpretation of these results in the light of current views of the respiratory chain is discussed.