Effectiveness of Coating Acrylic Resin Dentures on Preventing Candida Adhesion
Effectiveness of Coating Acrylic Resin Dentures on Preventing Candida Adhesion
复制标题
DOI：
10.1111/jopr.12046
复制
发表时间：
2013-08-01
影响因子：
4
通讯作者：
Suresh, C. S.
中科院分区：
文献类型：
--
作者：
Ali, Aiman A.;Alharbi, Fahad A.;Suresh, C. S.
目的：通过改良丙烯酸树脂义齿的表面，防止白色念珠菌在义齿上的粘附。材料与方法：90块丙烯酸树脂板分为三组。第一组：常规加工的丙烯酸树脂板。第二组：用2-氰基丙烯酸酯胶粘剂涂漆的板材。第三组：用Adper Single Bond Adhesive涂漆的板材。将所有标本分别浸入含有白色念珠菌的人工唾液容器中，在37℃下孵育11 d，对粘附念珠菌采用直接培养、载玻片计数和连续稀释三种评估方法进行计数。结果：1/10、1/10(2)、1/10(3)稀释组白色念珠菌过度生长，而III组只有1/10稀释组过度生长。对于III组，1/10(2)、1/10(3)、1/10(4)和1/10(5)稀释后的平均菌落数分别为123、22、3.4和0。关于载玻片计数，I组的平均真菌计数为166，而稀释1/10的组为40，稀释1/10(2)的组为21，稀释1/10(3)的组为8.6，稀释1/10(3)的组为0.7，稀释1/10(4)的组为1.2。无论采用何种评估方法，II组均未发现任何念珠菌菌落（0%）。这些差异具有统计学意义（p < 0.0001）。结论：Adper单键粘接剂涂覆丙烯酸树脂义齿可有效降低白色念珠菌对义齿的粘附，而氰基丙烯酸酯2-辛酯粘接剂涂覆可完全抑制白色念珠菌对义齿的粘附。
Purpose: The aim of this study was to prevent the adhesion of C. albicans on acrylic resin dentures by modifying their surfaces.Materials and Methods: Ninety acrylic resin plates were divided into three groups. Group I: conventionally processed acrylic resin plates. Group II: plates painted with 2-Octyl Cyanoacrylate adhesive. Group III: plates painted with Adper Single Bond Adhesive. All specimens were immersed separately in containers filled with artificial saliva that contained C. albicans and then incubated for 11 days at 37 degrees C. Three methods of evaluation were used to count the adhered Candida: direct culture, slide count, and serial dilutions.Results: C. albicans in 1/10, 1/10(2), and 1/10(3) dilutions showed overgrowth in group I, while overgrowth was noted only with 1/10 dilution in group III. For group III, mean colony numbers of 123, 22, 3.4, and 0 were found for the 1/10(2), 1/10(3), 1/10(4), and 1/10(5) dilutions, respectively. Regarding the slide counts, group I showed a mean fungal count of 166 compared to 40 for group III with 1/10 dilution, 21 compared to 9 with 1/10(2) dilution, 8.6 compared to 0.7 with 1/10(3) dilution, and 1.2 compared to 0 with 1/10(4) dilution. No plates in group II showed any candidal colonies regardless of the method of evaluation (0%). These differences were statistically significant (p < 0.0001).Conclusion: Coating the acrylic resin dentures with Adper Single Bond Adhesive was effective in reducing C. albicans adhesion to dentures, while coating with 2-Octyl Cyanoacrylate adhesive completely inhibited such adhesion.