Structure of the Enterococcus faecalis EIIA(gnt) PTS component.
Structure of the Enterococcus faecalis EIIA(gnt) PTS component.
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DOI：
10.1016/j.bbrc.2009.08.054
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发表时间：
2009-10
影响因子：
3.1
通讯作者：
Stefan Reinelt;B. Koch;M. Hothorn;W. Hengstenberg;S. Welti;K. Scheffzek
中科院分区：
文献类型：
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作者：
Stefan Reinelt;B. Koch;M. Hothorn;W. Hengstenberg;S. Welti;K. Scheffzek
在真细菌中，许多细胞外碳水化合物的利用是由糖特异性磷酸烯醇丙酮酸（PEP）依赖的糖磷酸转移酶系统（pts）介导的，该系统同时进口和磷酸化其目标糖。在这里，我们报道了迄今为止很少研究的粪肠球菌葡萄糖酸盐特异性PTS的eiiagnt组分的晶体结构。晶体结构显示出紧密相互作用的eiiagnt二聚体，其结构类似于来自大肠杆菌的相关eiiaman。对E. faecalis HPr、eiibmant及其与eiiamant复合物的同源性建模表明，尽管eiiamant和EIIAgnt之间的序列有一定的一致性，但活性位点与大肠杆菌系统中观察到的情况密切匹配，EIIAgnt的His-9可能是磷酸化基载体。因此，我们提出涉及eiiagnt的磷酸化转移反应根据类似于大肠杆菌EIIAman的机制进行。
In Eubacteria, the utilization of a number of extracellular carbohydrates is mediated by sugar specific phosphoenolepyruvate (PEP) dependent sugar phosphotransferase systems (PTSs), which simultaneously import und phosphorylate their target sugars. Here, we report the crystal structure of the EIIAgntcomponent of the so far little investigated Enterococcus faecalis gluconate specific PTS. The crystal structure shows a tightly interacting dimer of EIIAgntwhich is structurally similar to the related EIIAmanfrom Escherichia coli. Homology modeling of E. faecalis HPr, EIIBmanand their complexes with EIIAmansuggests that despite moderate sequence identity between EIIAmanand EIIAgnt, the active sites closely match the situation observed in the E. coli system with His-9 of EIIAgntbeing the likely phosphoryl group carrier. We therefore propose that the phosphoryl transfer reactions involving EIIAgntproceed according to a mechanism analog to the one described for E. coli EIIAman.