Decreased interstitial cell of Cajal volume in patients with slow-transit constipation
Decreased interstitial cell of Cajal volume in patients with slow-transit constipation
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DOI：
10.1016/s0016-5085(00)70409-4
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发表时间：
2000-01-01
期刊：
影响因子：
29.4
通讯作者：
Farrugia, G
中科院分区：
文献类型：
--
作者：
He, CL;Burgart, L;Farrugia, G
背景和目标：慢传输型便秘的病因尚不完全清楚，最近的观察表明Cajal间质细胞在控制肠道运动中起着重要作用。本研究的目的是确定正常乙状结肠和慢传输型便秘患者乙状结肠中Cajal间质细胞的体积。研究方法：乙状结肠样品用蛋白质基因产物9.5、c-Kit和α-平滑肌肌动蛋白的抗体染色。使用在激光扫描共聚焦显微镜和ANALYZE软件上收集的连续图像进行感兴趣区域的三维重建。结果如下：与正常对照组相比，慢传输型便秘患者乙状结肠标本各层Cajal间质细胞的体积显著减少。结肠环形平滑肌层内的神经元结构也减少。结论：Cajal间质细胞体积的减少可能在慢传输型便秘的病理生理中起重要作用。
Background & Aims: The cause of slow-transit constipation is incompletely understood, Recent observations suggest a central role for interstitial cells of Cajal in the control of intestinal motility. The aim of this study was to determine the volume of interstitial cells of Cajal in the normal sigmoid colon and in the sigmoid colon from patients with slow transit constipation. Methods: Sigmoid colonic samples were stained with antibodies to protein gene product 9.5, c-Kit, and alpha-smooth muscle actin, Three-dimensional reconstruction of regions of interest was performed using consecutive images collected on a laser scanning confocal microscope and ANALYZE software. Results: Volume of interstitial cells of Cajal was significantly decreased in all layers of sigmoid colonic specimens from patients with slow-transit constipation compared with normal controls. Neuronal structures within the colonic circular smooth muscle layer were also decreased. Conclusions: A decrease in the volume of interstitial cells of Cajal may play an important role in the pathophysiology of slow-transit constipation.