The organization and animal-vegetal asymmetry of cytokeratin filaments in stage VI Xenopus oocytes is dependent upon F-actin and microtubules
The organization and animal-vegetal asymmetry of cytokeratin filaments in stage VI Xenopus oocytes is dependent upon F-actin and microtubules
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DOI：
10.1006/dbio.1997.8508
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发表时间：
1997-04-01
影响因子：
2.7
通讯作者：
King, E
中科院分区：
文献类型：
--
作者：
Gard, DL;Cha, BJ;King, E
抗细胞角蛋白抗体的共聚焦免疫荧光显微镜显示了一个连续的和极化的细胞角蛋白（CK）丝在第六阶段爪蟾卵母细胞的皮质网络。在动物皮层中，CK细丝形成了致密的网状结构，其比先前在植物皮层中观察到的CK细丝的网络更厚并且表现出更细的网格（Kavikowsky等人，1987年）。CK丝在I期早期中期首次与卵母细胞的生发泡（GV）和线粒体质量（MM）联合出现，表明CK丝是三个细胞骨架网络中最后组装的。到后期I，CK细丝形成复杂的网络围绕GV，周围和穿透MM，并将这些网络连接到一个网状的CK细丝在卵母细胞皮层。在阶段M早期IV，CK丝形成了一个高度互连的，显然unpolarized，放射状阵列连接核周和皮质CK丝网络。在第四阶段中期至第五阶段，观察到CK微丝网络的极化，首先是动物，然后是植物CK微丝网络，采用了第六阶段卵母细胞的组织特征。用细胞松弛素B处理VI期卵母细胞，破坏皮质和细胞质CK丝的组织，从卵母细胞皮质释放CK丝，并诱导形成许多细胞质CK丝聚集体。CB还破坏了VI期卵母细胞中细胞质微管（MT）的组织。用诺考达唑处理卵母细胞MT导致皮质CK细丝网络的特征性A-V极性丧失。相反，通过显微注射抗CK抗体破坏细胞质CK丝对细胞质或MT组织没有明显影响。我们提出了一个模型，在该模型中的组织和极化的皮质网络的CR丝在第六阶段非洲爪蟾卵母细胞依赖于与肌动蛋白丝和微管的相互作用的层次结构。(C)北京：科学出版社.
Confocal immunofluorescence microscopy with anti-cytokeratin antibodies revealed a continuous and polarized network of cytokeratin (CK) filaments in the cortex of stage VI Xenopus oocytes. In the animal cortex, CK filaments formed a dense meshwork that both was thicker and exhibited a finer mesh than the network of CK filaments previously observed in the vegetal cortex (Klymkowsky et al., 1987). CK filaments first appeared in association with germinal vesicle (GV) and mitochondrial mass (MM) of oocytes in early mid stage I, indicating that CK filaments are the last of the three cytoskeletal networks to be assembled. By late stage I, CK filaments formed complex networks surrounding the GV, surrounding and penetrating the MM, and linking these networks to a meshwork of CK filaments in the oocyte cortex. During stage m-early IV, CK filaments formed a highly interconnected, apparently unpolarized, radial array linking the perinuclear and cortical CK filament networks. Polarization of the CK filament network was observed during mid stage IV-stage V, as first the animal, then the vegetal CK filament networks adopted the organization characteristic of stage VI oocytes. Treatment of stage VI oocytes with cytochalasin B disrupted the organization of both cortical and cytoplasmic CK filaments, releasing CK filaments from the oocyte cortex and inducing formation of numerous cytoplasmic CK filament aggregates. CB also disrupted the organization of cytoplasmic microtubules (MTs) in stage VI oocytes. Disassembly of oocyte MTs with nocodazole resulted in loss of the characteristic A-V polarity of the cortical CK filament network. In contrast, disruption of cytoplasmic CK filaments by microinjection of anti-CK antibodies had no apparent effect on cytoplasmic or MT organization. We propose a model in which the organization and polarization of the cortical network of CR filaments in stage VI Xenopus oocytes are dependent upon a hierarchy of interactions with actin filaments and microtubules. (C) 1997 Academic Press.