Increased association of telomerase with short telomeres in yeast
Increased association of telomerase with short telomeres in yeast
复制标题
DOI：
10.1101/gad.438907
复制
发表时间：
2007-07-15
影响因子：
10.5
通讯作者：
Shore, David
中科院分区：
文献类型：
--
作者：
Bianchi, Alessandro;Shore, David
端粒功能是由蛋白质复合物在端粒酶合成的端粒DNA（TG）重复序列阵列上组装介导的。染色体末端的端粒酶作用由端粒复合物精细调节，从而维持恒定的平均重复数。这是通过对TG束长度敏感的负反馈过程实现的，但其潜在机制尚不清楚。我们发现，短端粒，这是端粒酶的优先底物，显示增加关联的酶在细胞周期的S期，当端粒酶的行为。此外，我们提供了支持的分子机制，这一关键步骤的端粒酶招聘是由TG道长度调节。
Telomere function is mediated by the assembly of a protein complex on an array of telomeric DNA (TG) repeats synthesized by the telomerase enzyme. Telomerase action at chromosome ends is finely tuned by the telomeric complex so that a constant average number of repeats is maintained. This is achieved through a negative feedback process that is sensitive to TG tract length, but whose underlying mechanism is unknown. We show that short telomeres, which are preferential substrates for telomerase, display increased association with the enzyme in the S phase of the cell cycle, when telomerase acts. In addition, we provide support for a molecular mechanism by which this key step of telomerase recruitment is regulated by TG tract length.