Determination of capsaicinoids in Capsicum species using ultra performance liquid chromatography-mass spectrometry
Determination of capsaicinoids in Capsicum species using ultra performance liquid chromatography-mass spectrometry
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DOI：
10.1002/jssc.201200459
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发表时间：
2012-11-01
影响因子：
3.1
通讯作者：
Ahmed, Yacine Badjah Hadj
中科院分区：
文献类型：
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作者：
Alothman, Zeid Abdullah;Wabaidur, Saikh Mohammad;Ahmed, Yacine Badjah Hadj
在目前的工作中，提出了一种快速、灵敏的超高效液相色谱-质谱法用于分析不同辣椒样品中存在的辣椒素类化合物（去甲二氢辣椒素、辣椒素、二氢辣椒素、高辣椒素和高二氢辣椒素）。以乙醇为提取溶剂，采用液液萃取法提取辣椒素，采用反相C18柱，梯度流动相（溶剂A：乙腈，溶剂B：含0.1%甲酸的水）进行色谱分离。在最佳实验条件下，各辣椒素的线性范围为0.550 μg/g，相关系数（r2）>0.999，去甲二氢辣椒素、辣椒素、二氢辣椒素、高辣椒素和高二氢辣椒素的检出限分别为0.15、0.05、0.06、0.2和0.1 μg/g。具有相对标准偏差的方法的每次运行和每日精密度
In the present work, a rapid and sensitive ultra performance liquid chromatography-mass spectrometry method has been proposed for the analysis of capsaicinoids (nordihydrocapsaicin, capsaicin, dihydrocapsaicin, homocapsaicin, and homodihydrocapsaicin) present in different Capsicum samples. Extraction of capsaicinoids was carried out by liquidliquid extraction using ethanol as an extracting solvent, while the chromatographic separation was achieved by reversed phase C18 column with gradient mobile phase (solvent A: acetonitrile and solvent B: water with 0.1% formic acid). Under the optimum experimental conditions, the linear ranges were 0.550 mu g/g with correlation coefficient (r2) >0.999 for each capsaicinoids and detection limits were 0.15, 0.05, 0.06, 0.2, and 0.1 mu g/g for nordihydrocapsaicin, capsaicin, dihydrocapsaicin, homocapsaicin, and homodihydrocapsaicin, respectively. Run-to-run and day-to-day precisions of the method with relative standard deviations