Rapid HPLC determination of total homocysteine and other thiols in serum and plasma: sex differences and correlation with cobalamin and folate concentrations in healthy subjects.
Rapid HPLC determination of total homocysteine and other thiols in serum and plasma: sex differences and correlation with cobalamin and folate concentrations in healthy subjects.
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DOI：
10.1093/clinchem/40.6.873
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发表时间：
1994-06
影响因子：
9.3
通讯作者：
D. Jacobsen;V. Gatautis;R. Green;K. Robinson;S. R. Savon;M. Secic;Ji Ji-Ji;Joanne M. Otto;
中科院分区：
文献类型：
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作者：
D. Jacobsen;V. Gatautis;R. Green;K. Robinson;S. R. Savon;M. Secic;Ji Ji-Ji;Joanne M. Otto;
采用高效液相色谱-荧光检测技术快速测定了人血清和血浆中总同型半胱氨酸、半胱氨酸和半胱氨酸。我们早期的方法（Anal Biochem 1989;178:208）使用单溴茂烷特异性衍生硫醇，现已被广泛修改，以允许快速处理样品。因此，每天可检测80个样品的总同型半胱氨酸、半胱氨酸和半胱氨酸。该方法灵敏度高（检测下限<或= 4 pmol），准确度高（同型半胱氨酸的检测内、间CV分别为3.31%和4.85%）。健康男性供者血浆和血清平均总同型半胱氨酸浓度（分别为9.26和12.30 μ mol/L, P < 0.001）和健康女性供者血浆和血清平均总同型半胱氨酸浓度（分别为7.85和10.34 μ mol/L, P < 0.001）差异显著。总同型半胱氨酸在两性间的差异也很显著（血浆和血清P = 0.002）。半胱氨酸和半胱氨酸也存在类似的差异。我们发现血清钴胺素和总同型半胱氨酸在男性（P = 0.0102）和女性（P = 0.0174）中呈显著负相关。血清叶酸也与两性总同型半胱氨酸呈负相关。
High-performance liquid chromatography with fluorescence detection has been utilized for the rapid determination of total homocysteine, cysteine, and cysteinylglycine in human serum and plasma. Our earlier procedure (Anal Biochem 1989;178:208), which used monobromobimane to specifically derivatize thiols, has been extensively modified to allow for rapid processing of samples. As a result, > 80 samples a day can be assayed for total homocysteine, cysteine, and cysteinylglycine. The method is sensitive (lower limit of detection < or = 4 pmol in the assay) and precise (intra- and interassay CV for homocysteine, 3.31% and 4.85%, respectively). Mean total homocysteine concentrations in plasma and serum were significantly different, both from healthy male donors (9.26 and 12.30 mumol/L, respectively; P < 0.001) and healthy female donors (7.85 and 10.34 mumol/L, respectively; P < 0.001). The differences in total homocysteine between sexes were also significant (P = 0.002 for both plasma and serum). Similar differences were found for cysteine and cysteinylglycine. We found a significant inverse correlation between serum cobalamin and total homocysteine in men (P = 0.0102) and women (P = 0.0174). Serum folate also inversely correlated with total homocysteine in both sexes.