Spatial Control of Cell Adhesion and Patterning through Mussel-Inspired Surface Modification by Polydopamine
Spatial Control of Cell Adhesion and Patterning through Mussel-Inspired Surface Modification by Polydopamine
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DOI：
10.1021/la102825p
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发表时间：
2010-10-05
期刊：
影响因子：
3.9
通讯作者：
Park, Chan Beum
中科院分区：
文献类型：
--
作者：
Ku, Soak Hee;Lee, Joan Seok;Park, Chan Beum
通过使用贻贝启发的聚多巴胺（FDA）的通用粘附特性实现哺乳动物细胞的空间控制和图案化。多巴胺的自聚合，一个小分子的灵感来自贻贝脚蛋白的DOPA基序，导致在PDA吸附层的形成时，多巴胺水溶液连续注入到聚（二甲基硅氧烷）微通道。我们发现，各种细胞（纤维肉瘤小鼠前成骨细胞MC 3 T3-EI和小鼠成纤维细胞NIH-3 T3）主要粘附到PDA修饰的区域，保持其正常形态。细胞沿FDA条纹方向排列，这种趋势不受细胞系类型的限制。由于FDA修饰不需要复杂的化学反应，并且适用于任何类型的材料，因此与基于粘附蛋白固定化的传统方法相比，它能够以简单而通用的方式实现细胞图案化。基于FDA的细胞图案化方法应该在许多生物材料研究领域中是有用的，例如组织工程支架的制造、用于药物筛选的基于细胞的装置以及细胞材料相互作用的基础研究。
The spatial control and patterning of mammalian cells were achieved by using the universal adhesive property of mussel-inspired polydopamine (FDA). The self-polymerization of dopamine, a small molecule inspired by the DOPA motif of mussel foot proteins, resulted in the formation of a PDA adlayer when aqueous dopamine solution was continuously injected into poly(dimethylsiloxane) microchannels. We found that various cells (fibrosarcoma mouse preostcoblast MC3T3-EI, and mouse fibroblast NIH-3T3) predominantly adhered to PDA-modified regions, maintaining their normal morphologies. The cells aligned in the direction of striped FDA patterns, and this tendency was not limited by the type of cell line. Because FDA modification does not require complex chemical reactions and is applicable to any type of material, it enables cell patterning in a simple and versatile manner as opposed to conventional methods based on the immobilization of adhesive proteins. The FDA-based method of cell patterning should be useful in many biomaterial research areas such as the fabrication of tissue engineering scaffolds, cell-based devices for drug screening, and the fundamental study of cell material interactions.