High expression levels of receptor activator of nuclear factor-kappa B ligand associated with human chronic periodontitis are mainly secreted by CD4+ T lymphocytes.
High expression levels of receptor activator of nuclear factor-kappa B ligand associated with human chronic periodontitis are mainly secreted by CD4+ T lymphocytes.
复制标题
与人类慢性牙周炎相关的核因子κB配体受体激活剂的高表达主要由CD4 T淋巴细胞分泌。
DOI：
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
J. Gamonal
中科院分区：
文献类型：
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作者：
R. Vernal;N. Dutzan;Marcela Hernández;Sabrita Chandía;J. Puente;R. Léon;L. Garcia;I. del Valle;Augusto Silva;J. Gamonal
背景
慢性牙周炎是一种以牙槽骨破坏和牙齿缺失为特征的感染性疾病。核因子-κ B配体受体激活因子（Receptor activator of nuclear factor-kappa B ligand，RANKL）是一种破骨细胞生成性细胞因子，是破骨细胞寿命的中心调节因子，参与生理和病理性骨吸收。牙龈T细胞合成RANKL，导致分子局部失衡，导致牙周炎中可见的牙槽骨吸收。我们的研究旨在将RANKL水平与慢性牙周炎患者牙龈组织中存在的CD 4（+）T细胞活性相关联。
方法
对33例慢性牙周炎患者和20例健康对照者进行牙龈活检。将标本福尔马林固定并石蜡包埋用于实时逆转录聚合酶链反应（RT-PCR）和组织学分析，或组织消化处理用于细胞培养和流式细胞术分析。通过定量RT-PCR和酶联免疫吸附试验（ELISA）测定牙龈细胞培养上清液中RANKL mRNA和蛋白水平。通过流式细胞术定量牙龈白细胞。用流式细胞术和共聚焦显微镜分析RANKL和CD 4免疫反应性。
结果
牙周炎患者RANKL mRNA水平高于健康受试者，患者自发和脂多糖（LPS）和植物血凝素（PHA）刺激的RANKL合成也高于对照组。CD 4（+）T淋巴细胞是牙周炎患者牙龈组织中主要的浸润细胞。此外，通过双染色流式细胞术和共聚焦显微镜确定RANKL和CD 4（+）T细胞之间的关联。
结论
综上所述，这些数据表明，牙龈CD 4（+）T细胞是负责在人类慢性牙周炎患者中观察到的较高水平的RANKL的主要细胞。
BACKGROUND
Chronic periodontitis is an infectious disease characterized by alveolar bone destruction and teeth loss. Receptor activator of nuclear factor-kappa B ligand (RANKL) is an osteoclastogenic cytokine, a central regulatory factor in the osteoclast's lifespan, and a participant in physiological and pathological bone resorption. Gingival T cells synthesize RANKL, contributing to molecular local imbalance that entails the alveolar bone resorption seen in periodontitis. Our study was aimed at associating the levels of RANKL with the CD4(+) T-cell activity present in gingival tissues of chronic periodontitis patients.
METHODS
Gingival biopsies were obtained from 33 chronic periodontitis patients and 20 healthy controls. Specimens were either formalin fixed and paraffin embedded for real-time reverse transcription-polymerase chain reaction (RT-PCR) and histologic analysis or tissue digestion processed for cell culture and flow-cytometry analysis. RANKL mRNA and protein levels were determined by quantitative RT-PCR and enzyme-linked immunosorbent assay (ELISA) in gingival-cell culture supernatants. Gingival leukocytes were quantified by flow cytometry. RANKL and CD4 immunoreactivity were analyzed by flow cytometry and confocal microscopy.
RESULTS
RANKL mRNA levels were higher in patients with periodontitis than in healthy subjects, and spontaneous and lipopolysaccharide (LPS)- and phytohemagglutinin (PHA)-stimulated RANKL synthesis were higher also in patients than controls. CD4(+) T lymphocytes were the predominant infiltrate cell subset present in gingival tissues of periodontitis patients. Furthermore, an association between RANKL and CD4(+) T cells was determined by double-staining flow cytometry and confocal microscopy.
CONCLUSION
Taken together, these data demonstrate that gingival CD4(+) T cells are the main cells responsible for higher levels of RANKL observed in human chronic periodontitis patients.
影响因子：
15.9
作者：
Teng, YTA;Nguyen, H;Penninger, JM
通讯作者：
Penninger, JM