Fluorescent oligo(p-phenyleneethynylene) contained amphiphiles-encapsulated magnetic nanoparticles for targeted magnetic resonance and two-photon optical imaging in vitro and in vivo.
Fluorescent oligo(p-phenyleneethynylene) contained amphiphiles-encapsulated magnetic nanoparticles for targeted magnetic resonance and two-photon optical imaging in vitro and in vivo.
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DOI：
10.1039/c5nr00806a
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发表时间：
2015-05
期刊：
影响因子：
6.7
通讯作者：
Chao Yin;B. Hong;Zhaocui Gong;Hui Zhao;Wenbo Hu;Xiaomei Lu;Jie Li;Xiang Li;Zhen Yang
中科院分区：
文献类型：
--
作者：
Chao Yin;B. Hong;Zhaocui Gong;Hui Zhao;Wenbo Hu;Xiaomei Lu;Jie Li;Xiang Li;Zhen Yang
采用方便的疏水封装方法，成功制备了具有多模态成像能力的叶酸受体靶向多功能荧光磁性纳米颗粒（FMNPs）。核心的氧化铁纳米颗粒提供了t2加权磁共振成像（MRI），而纳米颗粒表面的两亲性低聚物具有良好的水溶性，生物相容性，优异的荧光特性和癌症靶向性。这些纳米粒子具有超顺磁性，饱和磁化强度（Ms）为23 emu g(-1)，横向弛豫率为140.89 mM(-1) s（-1）。体外研究表明，双峰态FMNPs可以作为有效的双光子荧光探针和磁探针，实现Hela细胞的靶向成像，且无明显的细胞毒性。体内双光子荧光和MRI结果表明，FMNPs能够优先在肿瘤组织中积累，从而实现活体肿瘤的双模检测。这些研究为开发用于多模态成像的新型多功能探针提供了思路，将在生物医学治疗学中发挥重要作用。
Folate receptor-targeted multifunctional fluorescent magnetic nanoparticles (FMNPs) composed of cores containing iron oxide nanocrystals and amphiphilic oligo(p-phenyleneethynylene) shells with multimodal imaging capability were successfully prepared through a convenient hydrophobic encapsulation approach. The iron oxide nanoparticles in the core provided T2-weighted magnetic resonance imaging (MRI), whereas the amphiphilic oligomers on the surface of the nanoparticles introduced good water-solubility, biocompatibility, excellent fluorescent properties and cancer-targeting. These nanoparticles exhibited superparamagnetic properties with saturation magnetization (Ms) of 23 emu g(-1) and a transverse relaxivity rate of 140.89 mM(-1) s(-1). In vitro studies indicated that the dual-modal FMNPs can serve as an effective two-photon fluorescent and a magnetic probe to achieve the targeted imaging of Hela cells without obvious cytotoxicity. In vivo two-photon fluorescence and MRI results demonstrated that the FMNPs were able to preferentially accumulate in tumor tissues to allow dual-modal detection of tumors in a living body. These studies provided insight in developing novel multifunctional probes for multimodal imaging, which would play an important role for theranostics in biomedical science.