Neutrophil extracellular traps induced by cigarette smoke activate plasmacytoid dendritic cells
Neutrophil extracellular traps induced by cigarette smoke activate plasmacytoid dendritic cells
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香烟烟雾诱导的中性粒细胞胞外陷阱激活浆细胞样树突状细胞
DOI：
10.1136/thoraxjnl-2016-209887
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发表时间：
2017-12-01
期刊：
影响因子：
10
通讯作者：
Zhong, Xiao-Ning
中科院分区：
文献类型：
--
作者：
Qiu, Shi-Lin;Zhang, Hui;Zhong, Xiao-Ning
背景中性粒细胞胞外陷阱（NETs）代表了中性粒细胞捕获、限制和消除入侵微生物的独特策略。新出现的证据表明，NET在没有微生物刺激的情况下对宿主产生有害影响。然而，NET在吸烟相关肺部疾病中的作用仍有待阐明。目的研究吸烟诱导的慢性炎症过程中神经内分泌细胞（NETs）的形成及其在实验性肺气肿小鼠模型中的作用。方法采用荧光显微镜观察香烟烟雾暴露小鼠体内神经内毒素的形成和降解。NET对浆细胞样树突状细胞的潜在影响也进行了研究。结果香烟烟雾暴露小鼠体内NETs更易由多形核粒细胞形成，但不能降解。香烟烟雾提取物（CSE）作为一个重要的促进剂，触发中性粒细胞进行NETosis在体外。此外，CSE诱导的NET能够驱动浆细胞样树突状细胞成熟和活化，从而启动T细胞介导的免疫应答。结论在香烟烟雾暴露诱导的慢性炎症条件下，NET可能代表了先天性和适应性免疫应答之间的关键联系。
Background Neutrophil extracellular traps (NETs) represent a distinct strategy by which neutrophils trap, confine and eliminate invading microorganisms. Emerging evidence suggests that NETs exert a deleterious effect to the host in the absence of microbial stimuli. However, the role of NETs in smoking-related lung diseases remains to be elucidated. Objectives To evaluate the formation of NETs in the context of chronic inflammation induced by cigarette smoking and explore its potential role in an experimental mouse model of emphysema. Methods The formation and degradation of NETs in cigarette smoke exposed mice was assessed with a fluorescence microscope. The potential influences of NETs on plasmacytoiddendritic cells were also investigated. Results NETs were more prone to formation by polymorphonuclearneutrophils but defective in degradation in cigarette smoke exposed mice. Cigarette smoke extract (CSE) served as an important facilitator that triggered neutrophils to undergo NETosis in vitro. Furthermore, CSE-induced NETs were capable of driving plasmacytoiddendritic cell maturation and activation, thereby initiating a T-cell-mediated immune response. Conclusions NETs may represent a critical connection between innate and adaptive immune responses under conditions of chronic inflammation induced by cigarette smoke exposure.