DTI Metrics Can Be Used as Biomarkers to Determine the Therapeutic Effect of Stem Cells in Acute Peripheral Nerve Injury
DTI Metrics Can Be Used as Biomarkers to Determine the Therapeutic Effect of Stem Cells in Acute Peripheral Nerve Injury
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DTI指标可作为生物标志物来确定干细胞在急性周围神经损伤中的治疗效果
DOI：
10.1002/jmri.25395
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发表时间：
2017-03-01
影响因子：
4.4
通讯作者：
Shen, Jun
中科院分区：
文献类型：
--
作者：
Chen, Yue-Yao;Zhang, Xiang;Shen, Jun
目的：探讨弥散张量成像(DTI)作为骨髓间充质干细胞(MSCs)治疗急性周围神经损伤的生物标志物的作用。材料和方法：44只成年大鼠在坐骨神经干挤压伤后1周接受松果体下微量注射MSCs(n=22)或磷酸盐缓冲盐水(PBS，n=22)。连续的脂肪抑制T2加权成像、T2测量、DTI和坐骨神经功能评估在3.0Tesla磁共振设备上进行了8周的随访，组织学评估定期进行。比较两组的坐骨神经功能指数、T2值和DTI指标，包括损伤神经远端的各向异性分数(FA)、轴向扩散系数、径向扩散系数(RD)和平均扩散系数。结果：与PBS治疗的神经相比，MSCs治疗的神经功能恢复更好，神经再生更明显。MSCs组和PBS组的T2值变化规律相似(P=0.174)，而MSCs组的FA和RD值比PBS组更快地恢复到基线水平(P=0.045；0.035)。术后2~3周，骨髓间充质干细胞移植神经的FA和RD值均高于PBS移植神经(P0.0001，0.004；P=0.004，0.006)。结论：弥散张量成像的FA和RD值可作为检测干细胞治疗急性周围神经挤压伤疗效的敏感生物标志物。
Purpose: To determine the role of diffusion tensor imaging (DTI) metrics as biomarkers for the therapeutic effects of mesenchymal stem cells (MSCs) in acute peripheral nerve injury.Materials and Methods: Forty-four adult rats received subepineurial microinjection of MSCs (n=22) or phosphate buffered saline (PBS, n=22) 1 week after the sciatic nerve trunk crush injury. Sequential fat-suppressed T2-weighted imaging, T2 measurement, DTI and sciatic nerve functional assessment were performed at a 3.0 Tesla MR unit over an 8-week follow-up, with histological assessments performed at regular intervals. The sciatic nerve function index, T2 value, and DTI metrics, including fractional anisotropy (FA), axial diffusivity, radial diffusivity (RD), and mean diffusivity values of the distal stumps of crushed nerves were measured and compared between the two groups.Results: Nerves treated with MSCs showed better functional recovery and exhibited more pronounced nerve regeneration compared with nerves treated with PBS. T2 values in nerves treated with MSCs or PBS showed a similar change pattern (P=0.174), while FA and RD values in nerves treated with MSCs showed more rapid return (one week earlier) to baseline level than nerves treated with PBS (P=0.045; 0.035). Nerves treated with MSCs had higher FA and lower RD values than nerves treated with PBS during the period from 2 to 3 weeks after surgery (P0.0001, 0.004; P=0.004, 0.006).Conclusion: FA and RD values derived from DTI might be used as sensitive biomarkers for detecting the therapeutic effect of stem cells in acute peripheral nerve crush injuries.