A Database of microRNA Expression Patterns in Xenopus laevis.
A Database of microRNA Expression Patterns in Xenopus laevis.
复制标题
DOI：
10.1371/journal.pone.0138313
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Wheeler GN
中科院分区：
文献类型：
--
作者：
Ahmed A;Ward NJ;Moxon S;Lopez-Gomollon S;Viaut C;Tomlinson ML;Patrushev I;Gilchrist MJ;Dalmay T;Dotlic D;Münsterberg AE;Wheeler GN
微小RNA（miRNA）是一种短的非编码RNA，长约22个核苷酸。它们通过翻译抑制或通过引起它们结合的mRNA的降解来抑制基因表达。许多是高度保守的不同的生物体和有限的时空表达模式在胚胎发育过程中，他们被认为是参与产生准确的发育时间和支持细胞命运的决定和组织的身份。我们使用LNA寡核苷酸确定了非洲爪蟾胚胎中180种miRNA的表达模式。此外，我们对非洲爪蟾早期发育的不同阶段进行了小RNA-seq，鉴定了属于29个新家族的44种miRNA，并表征了其中5种的表达。我们的分析确定了发育胚胎的许多器官中的miRNA表达。特别是在神经组织和体节中大量表达。令人惊讶的是，我们所研究的miRNAs都没有在心脏中表达。我们的结果已作为XenMARK和Xenbase中的资源免费提供。
MicroRNAs (miRNAs) are short, non-coding RNAs around 22 nucleotides long. They inhibit gene expression either by translational repression or by causing the degradation of the mRNAs they bind to. Many are highly conserved amongst diverse organisms and have restricted spatio-temporal expression patterns during embryonic development where they are thought to be involved in generating accuracy of developmental timing and in supporting cell fate decisions and tissue identity. We determined the expression patterns of 180 miRNAs in Xenopus laevis embryos using LNA oligonucleotides. In addition we carried out small RNA-seq on different stages of early Xenopus development, identified 44 miRNAs belonging to 29 new families and characterized the expression of 5 of these. Our analyses identified miRNA expression in many organs of the developing embryo. In particular a large number were expressed in neural tissue and in the somites. Surprisingly none of the miRNAs we have looked at show expression in the heart. Our results have been made freely available as a resource in both XenMARK and Xenbase.