Molecular identification and characterization of novel members of the human organic anion transporter (OATP) family
Molecular identification and characterization of novel members of the human organic anion transporter (OATP) family
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DOI：
10.1006/bbrc.2000.2922
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发表时间：
2000-06-24
影响因子：
3.1
通讯作者：
Tsuji, A
中科院分区：
文献类型：
--
作者：
Tamai, I;Nezu, J;Tsuji, A
我们鉴定了三种结构上属于人类有机阴离子转运多肽（OATP）家族的新型转运蛋白。由于先前已知的大鼠oatp1至3在组织分布或功能方面不一定对应于人类OATP，因此在这里我们将新鉴定的人类OATP指定为OATP-B、-D和-E，并将先前已知的人类OATP重命名为OATP-A。 OATP-C 被证明与最近报道的 LST1/OATP-2 相同，五种 OATP 和前列腺素转运蛋白 PGT（OATP 家族成员）在人体组织中的表达谱表明，OATP-C 仅定位于肝脏，OATP-A 和 PGT 在有限范围的组织中表达，OATP-B、-D 和 -E 显示广泛的表达谱。 OATP-B、-C、-D 和 -E 对 [H-3]雌酮-3-硫酸盐作为常见底物表现出转运活性。 OATP-C 具有高转运活性和广泛的底物特异性。 (C) 2000 年学术出版社。
We identified three novel transporters structurally belonging to the organic anion transporting polypeptide (OATP) family in humans. Since previously known rat oatp1 to 3 do not necessarily correspond to the human OATPs in terms of either tissue distribution or function, here we designate the newly identified human OATPs as OATP-B, -D and -E, and we rename the previously known human OATP as OATP-A. OATP-C proved to be identical with the recently reported LST1/OATP-2, Expression profiles of the five OATPs and the prostaglandin transporter PGT (a member of OATP family) in human tissues showed that OATP-C is exclusively localized in liver, OATP-A and PGT are expressed in restricted ranges of tissues, and OATP-B, -D and -E show broad expression profiles. OATP-B, -C, -D and -E exhibited transport activity for [H-3]estrone-3-sulfate as a common substrate. OATP-C has a high transport activity with broad substrate specificity. (C) 2000 Academic Press.