QUANTITATIVE-ANALYSIS OF RNA PRODUCED BY SLOW AND FAST ALLELES OF ADH IN DROSOPHILA-MELANOGASTER
QUANTITATIVE-ANALYSIS OF RNA PRODUCED BY SLOW AND FAST ALLELES OF ADH IN DROSOPHILA-MELANOGASTER
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DOI：
10.1073/pnas.85.14.5161
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发表时间：
1988-07-01
影响因子：
11.1
通讯作者：
STAM, LF
中科院分区：
文献类型：
--
作者：
LAURIE, CC;STAM, LF
黑腹果蝇的乙醇脱氢酶（ADH）基因座（ADH）基因座（Adh）在全球范围内对于两种同种酶（快和慢）是多态性的。本研究旨在确定等位酶之间 ADH 蛋白浓度的明确差异是否是由于 mRNA 水平的差异所致。 RNA 凝胶印迹杂交和 RNase 保护测定用于量化 ADH mRNA 水平。每种方法均使用 Adh 无效突变体作为内标。分析了不同地理起源的几个慢速和快速等位基因对。结果提供了强有力的证据，表明 ADH 蛋白浓度差异不是由 RNA 水平解释的。
The alcohol dehydrogenase (ADH) locus (ADH) locus (Adh) of Drosophila melanogaster is polymorphic on a worldwide basis for two allozymes, Fast and Slow. This study was undertaken to determine whether the well-established difference in ADH protein concentration between the allozymes is due to a difference in mRNA levels. RNA gel blot hydridization and an RNase protection assay were used to quantify ADH mRNA levels. Each method used an Adh null mutant as an internal standard. Several Slow and Fast allele pairs of different geographic origins were analyzed. The results provide strong evidence that the ADH protein concentration difference is not accounted for by RNA level.