Aluminum induces a decrease in cytosolic calcium concentration in BY-2 tobacco cell cultures
Aluminum induces a decrease in cytosolic calcium concentration in BY-2 tobacco cell cultures
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DOI：
10.1104/pp.116.1.81
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发表时间：
1998-01-01
期刊：
影响因子：
7.4
通讯作者：
Gilroy, S
中科院分区：
文献类型：
--
作者：
Jones, DL;Kochian, LV;Gilroy, S
铝毒性是限制酸性土壤作物生产力的一个主要问题。有人认为，Al 毒性与细胞 Ca 稳态的变化和质膜 Ca2+ 通透通道的阻塞有关。烟草 (Nicotiana tabacum L.) 的 BY-2 悬浮培养细胞表现出对铝敏感的快速细胞扩增。因此，在 BY-2 细胞中追踪 Al 对细胞质游离 Ca 浓度 ([Ca2+](cyt)) 变化的影响，以评估 Al 是否扰乱细胞 Ca 稳态。 Al 暴露导致 [Ca2+](cyt) 长期减少和生长抑制，类似于 Ca2+ 通道阻滞剂 La3+ 和 Ca2+ 螯合剂乙二醇-双（β-氨基乙基醚）-N,N'-四乙酸的效果。 Ca2+ 通道阻滞剂维拉帕米和硝苯地平不会引起这些细胞中 [Ca2+](cyt) 的减少，也无法抑制生长。 Al和La3+，而非维拉帕米或硝苯地平，降低了Indo-1荧光的Mn2+猝灭速率，这与Ca2+-和Mn2+-通透通道的阻塞一致。这些结果表明，Al 可能会阻断植物细胞质膜上的 Ca2+ 通道，并且这种作用可能在 Al 离子的植物毒性活性中发挥至关重要的作用。
Al toxicity is a major problem that limits crop productivity on acid soils. It has been suggested that Al toxicity is linked to changes in cellular Ca homeostasis and the blockage of plasma membrane Ca2+-permeable channels. BY-2 suspension-cultured cells of tobacco (Nicotiana tabacum L.) exhibit rapid cell expansion that is sensitive to Al. Therefore, the effect of Al on changes in cytoplasmic free Ca concentration ([Ca2+](cyt)) was followed in BY-2 cells to assess whether Al perturbed cellular Ca homeostasis. Al exposure resulted in a prolonged reduction in [Ca2+](cyt) and inhibition of growth that was similar to the effect of the Ca2+ channel blocker La3+ and the Ca2+ chelator ethyleneglycol-bis(beta-aminoethyl ether)-N,N'-tetraacetic acid. The Ca2+ channel blockers verapamil and nifedipine did not induce a decrease in [Ca2+](cyt) in these cells and also failed to inhibit growth. Al and La3+, but not verapamil or nifedipine, reduced the rate of Mn2+ quenching of Indo-1 fluorescence, which is consistent with the blockage of Ca2+- and Mn2+-permeable channels. These results suggest that Al may act to block Ca2+ channels at the plasma membrane of plant cells and this action may play a crucial role in the phytotoxic activity of the Al ion.