Developmental and tissue-specific expression of CHS1 from Plutella xylostella and its response to chlorfluazuron
Developmental and tissue-specific expression of CHS1 from Plutella xylostella and its response to chlorfluazuron
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DOI：
10.1016/j.pestbp.2007.02.004
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发表时间：
2007-09-01
影响因子：
4.7
通讯作者：
Tsumuki, Hisaaki
中科院分区：
文献类型：
--
作者：
Ashfaq, Muhammad;Sonoda, Shoji;Tsumuki, Hisaaki
采用RT-PCR和实时定量PCR技术（qPCR）分析了小菜蛾（Plutella xylostella, PxCHS1）几丁质合成酶-1基因的cDNA，并研究了PxCHS1A和PxCHS1B两个剪接变体在不同发育阶段和主要身体组织中的表达规律。PxCHS1 cDNA全长5461 bp，开放阅读框4701 bp，编码1567个氨基酸，预计分子量为179 kDa。这两个剪接变体是由大小相同（177 bp）的互斥外显子表达的，但在核苷酸水平上只有66%的同源性。两种剪接变异体在所有发育阶段均有表达。qPCR数据显示，这两种变体在体内表达不均匀，其中PxCHS1A的表达量比PxCHS1B高3.7倍。PxCHS1A在头部的表达量比身体高1.5倍，而PxCHS1B在头部和身体的表达量相差6.3倍。氯氟脲未改变PxCHS1在幼虫体内的表达。(C) 2007爱思唯尔公司版权所有。
The cDNA of chitin synthase-1 gene of Plutella xylostella (PxCHS1) was characterized and expression patterns of the two splice variants PxCHS1A and PxCHS1B were investigated in various developmental stages and in major body tissues by RT-PCR and real-time quantitative PCR (qPCR). The PxCHS1 cDNA was 5461 bp in length with an open reading frame of 4701 bp that encoded a putative protein of 1567 amino acids with predicted molecular mass of 179 kDa. The two splice variants were expressed from the mutually exclusive exons which were same in size (177 bp) but showed only 66% identity at the nucleotide level. Both splice variants were expressed in all developmental stages. The qPCR data suggested an uneven expression of the two variants in the body where expression of PxCHS1A was 3.7-fold higher than that of PxCHS1B. The expression of PxCHS1A was 1.5-fold higher in the head than the body whereas in case of PxCHS1B the difference between head and body was 6.3-fold. Chlorfluazuron did not change the expression of PxCHS1 in larvae. (C) 2007 Elsevier Inc. All rights reserved.