Improvement of bacterial blight and brown planthopper resistance in an elite restorer line Huazhan of Oryza
Improvement of bacterial blight and brown planthopper resistance in an elite restorer line Huazhan of Oryza
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DOI：
10.1016/j.fcr.2015.11.009
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发表时间：
2016-02-01
影响因子：
5.8
通讯作者：
Xiao, Guoying
中科院分区：
文献类型：
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作者：
Xiao, Youlun;Li, Jinjiang;Xiao, Guoying
培育抗病品种是防治水稻白叶枯病和褐飞虱这两种主要害虫最经济有效的策略之一。杂交水稻天优花占（天丰A/花占）是我国水稻生产上广泛应用的品种，但该杂交种对稻瘟病和褐飞虱均感病。本研究采用标记辅助回交（MABC）结合表型选择的方法，将1个抗稻瘟病基因（Xa 23）和2个抗褐飞虱基因（Bph 14和Bph 15）同时导入天优花占，以提高天优花占对稻瘟病和褐飞虱的抗性。抗BB和BPH鉴定结果表明，几乎所有改良恢复系及其衍生的杂交种均表现高抗或抗BB和BPH。田间试验表明，改良恢复系及其杂交种的农艺性状与各自的原始版本基本一致，但改良杂交种的千粒重比天优化占有极显著的提高。2013年和2014年小区产量试验结果表明，田丰A/R43-06和田丰A/R43-07两个改良组合的小区产量均高于天优花占等改良组合。这些结果表明，Xa 23、Bph 14和Bph 15基因的缺失是改良BB和BPH抗性的有效途径，两个杂交组合天丰A/R43-06和天丰A/R43-07可替代天优化占在我国BB和/或BPH流行区推广。(C)2015 Elsevier B. V.版权所有。
Development of resistant varieties is one of the most economical and effective strategies to prevent rice from bacterial blight disease (BB) and brown planthopper (BPH), the two main pests jeopardizing rice production. The hybrid rice Tianyouhuazhan (TianfengA/Huazhan) has been widely used in rice production in China, but this hybrid is susceptible to BB and BPH. In this study, one BB resistance gene (Xa23) and two BPH resistance genes (Bph14 and Bph15) were simultaneously introgressed into the restorer line Huazhan to improve the BB and BPH resistance of Tianyouhuazhan using marker -assisted backcrossing (MABC) strategy coupled with phenotypic selection. The results of identification of BB and BPH resistance revealed that almost all of the improved restorer lines and their derived hybrids showed high resistance (HR) or resistance (R) to BB and BPH. Almost all of the agronomic traits of improved restorer lines and their hybrids were similar to those of their respective original versions in field trial, except the 1000 -grain weight of improved hybrids showed an extremely significant increase than that of Tianyouhuazhan. Plot yields test in 2013 and 2014 suggested that two improved hybrid combinations named as TianfengA/R43-06 and TianfengA/R43-07 had higher grain yields per plot than Tianyouhuazhan and other improved hybrids. These results clearly indicate that pyramiding of Xa23, Bph14 and Bph15 genes is a useful approach for improving BB and BPH resistance, two hybrid combinations TianfengA/R43-06 and TianfengA/R43-07 could replace Tianyouhuazhan for extension in BB and/or BPH epidemic area in China. (C) 2015 Elsevier B.V. All rights reserved.