Never in mitosis gene A-related kinase 6 and aurora kinase A: New gene biomarkers in the conversion from ulcerative colitis to colorectal cancer
Never in mitosis gene A-related kinase 6 and aurora kinase A: New gene biomarkers in the conversion from ulcerative colitis to colorectal cancer
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DOI：
10.3892/or.2015.4187
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发表时间：
2015-10-01
期刊：
影响因子：
4.2
通讯作者：
Korkmaz, Mehmet
中科院分区：
文献类型：
--
作者：
Gerceker, Emre;Boyacioglu, Seda Orenay;Korkmaz, Mehmet
溃疡性结肠炎（UC）是结直肠癌（CRC）的重要危险因素。组蛋白修饰是表观遗传机制之一，可能在结直肠癌的致癌过程中发挥关键作用。目前文献中还没有比较UC和CRC组蛋白修饰模式的研究。因此，本研究的目的是调查基因，特别是那些参与组蛋白修饰的基因，对于监测 UC 中 CRC 发展的患者是否有价值。使用 RT-PCR 阵列技术评估和比较 CRC、UC 和对照组组蛋白修饰酶的关键基因表达。根据疾病的程度和持续时间以及炎症负荷将患者分为亚组，这些被认为是 UC 患者发生 CRC 的危险因素。在 UC 和 CRC 组中，与对照组相比，NEK6 和 AURKA 基因的过度表达显着升高。此外，UC组中HDAC1和PAK1基因的过表达显着较高，CRC组中HDAC1、HDAC7、PAKI和AURKB基因的过表达显着较高。 NEK6、AURKA、HDAC1 和 PAK1 在 UC 病程较长的患者中显着过度表达。 AURKA 和 NEK6 基因的过度表达在结肠受累更广泛的 UC 患者中更为明显。 HDAC1、HDAC7、PAK1、NEK6、AURKA 和 AURKB 是结直肠癌发生过程中重要的诊断和预后标志物。 HDAC1、PAK1、NEK6 和 AURKA 可被视为诊断标志物，用于 UC 患者的 CRC 筛查。
Ulcerative colitis (UC) is an important risk factor for colorectal cancer (CRC). Histone modifications are one of the epigenetic mechanisms that may have key roles in the carcinogenesis of CRC. At present, there are no studies comparing histone modification patterns of UC and CRC in the literature. Therefore the aim of the present study was to investigate whether genes, particularly those involved in histone modification, have value in patient monitoring with regards to CRC development in UC. Key gene expressions of the histone modification enzyme were assessed and compared in CRC, UC and control groups using the RT-PCR array technique. Patients were divided into subgroups based on the extent and duration of the disease and inflammatory burden, which are considered risk factors for CRC development in UC patients. In UC and CRC groups, a significantly higher overexpression of the NEK6 and AURKA genes compared to the control group was identified. In addition, there was a significantly higher overexpression of HDAC1 and PAK1 genes in the UC group, and of HDAC1, HDAC7, PAKI and AURKB genes in the CRC group. NEK6, AURKA, HDAC1 and PAK1 were significantly overexpressed in patients with a longer UC duration. Overexpression of AURKA and NEK6 genes was significantly more pronounced in UC patients with more extensive colon involvement. HDAC1, HDAC7, PAK1, NEK6, AURKA and AURKB are important diagnostic and prognostic markers involved in the carcinogenesis of CRC. HDAC1, PAK1, NEK6 and AURKA may be considered as diagnostic markers to be used in CRC screening for UC patients.